Potempa L A, Siegel J N, Gewurz H
J Immunol. 1981 Oct;127(4):1509-14.
C-reactive protein previously was shown to selectively and reversibly precipitate with certain small polymers of arginine and lysine. In the present report, calcium was shown to inhibit this reactivity in a dose-dependent manner, in direct contrast to the requirement for calcium for precipitation of CRP with C-polysaccharide. However, in the presence of phosphocholine, CRP rapidly precipitated and formed stable complexes with the polycationic polymers in the otherwise inhibitory calcium concentrations. alpha-Glycerol-phosphocholine, unlike phosphocholine, did not reverse this inhibitory effect. These results extend the characterization of the binding reactivity of CRP for polycations and suggest a relationship between this binding site and the sites for calcium and phosphocholine. We propose that CRP-polycation interactions in the presence of phosphocholine may have physiologic significance during the acute inflammatory process.
先前已表明，C反应蛋白可与某些精氨酸和赖氨酸的小聚合物选择性且可逆地沉淀。在本报告中，已表明钙以剂量依赖性方式抑制这种反应性，这与C反应蛋白与C多糖沉淀需要钙形成直接对比。然而，在磷酸胆碱存在的情况下，C反应蛋白迅速沉淀，并在原本具有抑制作用的钙浓度下与聚阳离子聚合物形成稳定的复合物。与磷酸胆碱不同，α-甘油磷酸胆碱不会逆转这种抑制作用。这些结果扩展了C反应蛋白对聚阳离子结合反应性的特征，并表明该结合位点与钙和磷酸胆碱的位点之间存在关联。我们提出，在急性炎症过程中，磷酸胆碱存在下的C反应蛋白-聚阳离子相互作用可能具有生理意义。
