在三氟乙醇存在的情况下，β-乳球蛋白非天然α-螺旋形式的两相诱导。
Two-phase induction of the nonnative alpha-helical form of beta-lactoglobulin in the presence of trifluoroethanol.
作者信息
Mendieta J, Folqué H, Tauler R
机构信息
Departament de Química Analítica, Universitat de Barcelona, 08028-Barcelona,
出版信息
Biophys J. 1999 Jan;76(1 Pt 1):451-7. doi: 10.1016/S0006-3495(99)77212-7.
Abstract
The trifluoroethanol-dependent induction of the nonnative alpha-helical form of beta-lactoglubulin has been studied by circular dichroism spectroscopy. Data analysis is performed by factor analysis and multivariate curve resolution. An intermediate form in the induction of the alpha-helical form of the beta-lactoglobulin has been identified at low TFE concentration. By application of an alternating least-squares algorithm, the CD spectrum corresponding to the intermediate form has been resolved. The deconvolution of this CD spectrum shows a secondary structure content more in agreement with the one predicted from the amino acid sequence than the secondary structure of the helical form obtained at higher TFE concentrations. The additional alpha-helical content of the form present at higher TFE concentrations could be due to nonspecific interaction of TFE with the polypeptide chain.
摘要
通过圆二色光谱法研究了三氟乙醇依赖性诱导β-乳球蛋白非天然α-螺旋形式的过程。数据分析采用因子分析和多元曲线分辨法。在低三氟乙醇浓度下，已鉴定出β-乳球蛋白α-螺旋形式诱导过程中的一种中间形式。通过应用交替最小二乘法，解析出了对应于中间形式的圆二色光谱。该圆二色光谱的去卷积显示，其二级结构含量与根据氨基酸序列预测的二级结构更为一致，而非在较高三氟乙醇浓度下获得的螺旋形式的二级结构。在较高三氟乙醇浓度下存在的形式中额外的α-螺旋含量可能是由于三氟乙醇与多肽链的非特异性相互作用所致。
相似文献
1
Two-phase induction of the nonnative alpha-helical form of beta-lactoglobulin in the presence of trifluoroethanol.在三氟乙醇存在的情况下，β-乳球蛋白非天然α-螺旋形式的两相诱导。
Biophys J. 1999 Jan;76(1 Pt 1):451-7. doi: 10.1016/S0006-3495(99)77212-7.
2
Trifluoroethanol-induced stabilization of the alpha-helical structure of beta-lactoglobulin: implication for non-hierarchical protein folding.三氟乙醇诱导β-乳球蛋白α-螺旋结构的稳定：对非层次蛋白质折叠的启示
J Mol Biol. 1995 Jan 13;245(2):180-94. doi: 10.1006/jmbi.1994.0015.
3
The equilibrium intermediate of beta-lactoglobulin with non-native alpha-helical structure.具有非天然α-螺旋结构的β-乳球蛋白平衡中间体。
J Mol Biol. 1997 Jun 20;269(4):479-87. doi: 10.1006/jmbi.1997.1055.
4
Intermolecular beta-sheet results from trifluoroethanol-induced nonnative alpha-helical structure in beta-sheet predominant proteins: infrared and circular dichroism spectroscopic study.分子间β-折叠源于三氟乙醇诱导的β-折叠主导蛋白中的非天然α-螺旋结构：红外和圆二色光谱研究
Arch Biochem Biophys. 1998 Jul 15;355(2):275-81. doi: 10.1006/abbi.1998.0718.
5
Trifluoroethanol-induced beta --> alpha transition in beta-lactoglobulin: hydration and cosolvent binding studied by 2H, 17O, and 19F magnetic relaxation dispersion.三氟乙醇诱导β-乳球蛋白的β→α转变：通过2H、17O和19F磁共振弛豫色散研究水合作用和共溶剂结合
Biochemistry. 2003 Nov 25;42(46):13708-16. doi: 10.1021/bi035330l.
6
Equilibrium unfolding CD studies of bovine beta-lactoglobulin and its 14-52 fragment at acidic pH.牛β-乳球蛋白及其14-52片段在酸性pH条件下的平衡去折叠圆二色光谱研究。
Biopolymers. 1999 May;49(6):441-50. doi: 10.1002/(SICI)1097-0282(199905)49:6<441::AID-BIP2>3.0.CO;2-A.
7
High helical propensity of the peptide fragments derived from beta-lactoglobulin, a predominantly beta-sheet protein.源自β-乳球蛋白（一种主要为β-折叠结构的蛋白质）的肽片段具有高螺旋倾向。
J Mol Biol. 1995 Dec 8;254(4):737-46. doi: 10.1006/jmbi.1995.0651.
8
Detection of the equilibrium folding intermediate of beta-lactoglobulin in the presence of trifluoroethanol by mass spectrometry.通过质谱法检测在三氟乙醇存在下β-乳球蛋白的平衡折叠中间体
Rapid Commun Mass Spectrom. 2007;21(6):1049-52. doi: 10.1002/rcm.2940.
9
Fluoroalcohol-induced stabilization of the alpha-helical intermediates of lentil lectin: implication for non-hierarchical lectin folding.氟代醇诱导的小扁豆凝集素α-螺旋中间体的稳定：对非层次凝集素折叠的影响
Arch Biochem Biophys. 2004 Nov 15;431(2):215-23. doi: 10.1016/j.abb.2004.07.029.
10
alpha-->beta transition of beta-lactoglobulin as evidenced by heteronuclear NMR.通过异核核磁共振证实的β-乳球蛋白的α向β转变。
J Mol Biol. 1998 Nov 6;283(4):731-9. doi: 10.1006/jmbi.1998.2117.
引用本文的文献
1
Denaturation and solvent effect on the conformation and fibril formation of TGFBIp.变性和溶剂对TGFBIp构象及纤维形成的影响
Mol Vis. 2009 Dec 8;15:2617-26.
2
Unfolding kinetics of beta-lactoglobulin induced by surfactant and denaturant: a stopped-flow/fluorescence study.表面活性剂和变性剂诱导的β-乳球蛋白的去折叠动力学：停流/荧光研究
Biophys J. 2007 Nov 15;93(10):3601-12. doi: 10.1529/biophysj.106.101667. Epub 2007 Aug 10.
3
Alpha-helix formation in melittin and beta-lactoglobulin A induced by fluorinated dialcohols.氟化二元醇诱导蜂毒肽和β-乳球蛋白A形成α-螺旋结构
J Phys Chem B. 2006 Jun 8;110(22):10903-9. doi: 10.1021/jp056124l.
4
Conformational transitions in beta-lactoglobulin induced by cationic amphiphiles: equilibrium studies.阳离子两亲物诱导的β-乳球蛋白构象转变：平衡研究
Biophys J. 2004 Apr;86(4):2392-402. doi: 10.1016/S0006-3495(04)74296-4.
5
Ultrasonic studies of alcohol-induced transconformation in beta-lactoglobulin: the intermediate state.β-乳球蛋白中酒精诱导的构象转变的超声研究：中间状态
Biophys J. 2003 Dec;85(6):3928-34. doi: 10.1016/S0006-3495(03)74806-1.
6
Lifetimes of intermediates in the beta -sheet to alpha -helix transition of beta -lactoglobulin by using a diffusional IR mixer.使用扩散红外混合器研究β-乳球蛋白从β-折叠向α-螺旋转变过程中中间体的寿命
Proc Natl Acad Sci U S A. 2001 Jun 5;98(12):6646-9. doi: 10.1073/pnas.101122898. Epub 2001 May 22.
7
The methanol-induced conformational transitions of beta-lactoglobulin, cytochrome c, and ubiquitin at low pH: a study by electrospray ionization mass spectrometry.低pH条件下甲醇诱导的β-乳球蛋白、细胞色素c和泛素的构象转变：电喷雾电离质谱研究
J Am Soc Mass Spectrom. 2001 Mar;12(3):317-28. doi: 10.1016/s1044-0305(00)00226-9.
本文引用的文献
1
Multivariate curve resolution: a possible tool in the detection of intermediate structures in protein folding.多元曲线分辨：蛋白质折叠过程中检测中间结构的一种可能工具。
Biophys J. 1998 Jun;74(6):2876-88. doi: 10.1016/S0006-3495(98)77994-9.
2
A soft-modeling approach to interpret thermodynamic and conformational transitions of polynucleotides.一种用于解释多核苷酸热力学和构象转变的软建模方法。
Biophys J. 1997 Dec;73(6):2937-48. doi: 10.1016/S0006-3495(97)78322-X.
3
A model for the interaction of trifluoroethanol with peptides and proteins.三氟乙醇与肽和蛋白质相互作用的模型。
Int J Pept Protein Res. 1996 Oct;48(4):328-36. doi: 10.1111/j.1399-3011.1996.tb00849.x.
4
Methods to estimate the conformation of proteins and polypeptides from circular dichroism data.从圆二色性数据估算蛋白质和多肽构象的方法。
Anal Biochem. 1996 Mar 1;235(1):1-10. doi: 10.1006/abio.1996.0084.
5
Non-native alpha-helical intermediate in the refolding of beta-lactoglobulin, a predominantly beta-sheet protein.β-乳球蛋白（一种主要为β-折叠结构的蛋白质）重折叠过程中的非天然α-螺旋中间体。
Nat Struct Biol. 1996 Oct;3(10):868-73. doi: 10.1038/nsb1096-868.
6
2,2,2-Trifluoroethanol induces helical conformation in an all beta-sheet protein.2,2,2-三氟乙醇可诱导全β-折叠蛋白形成螺旋构象。
Biochem Biophys Res Commun. 1996 May 6;222(1):33-7. doi: 10.1006/bbrc.1996.0693.
7
Folding of beta-sheet proteins.β-折叠蛋白质的折叠
Curr Opin Struct Biol. 1995 Aug;5(4):482-7. doi: 10.1016/0959-440x(95)80032-8.
8
Molten globule of cytochrome c studied by small angle X-ray scattering.通过小角X射线散射研究细胞色素c的熔球态
J Mol Biol. 1993 Feb 5;229(3):591-6. doi: 10.1006/jmbi.1993.1064.
9
Local and nonlocal interactions in globular proteins and mechanisms of alcohol denaturation.球状蛋白质中的局部和非局部相互作用以及酒精变性机制。
Protein Sci. 1993 Dec;2(12):2050-65. doi: 10.1002/pro.5560021206.
10
Equilibrium folding studies of cellular retinoic acid binding protein, a predominantly beta-sheet protein.细胞视黄酸结合蛋白（一种主要为β-折叠的蛋白质）的平衡折叠研究
Biochemistry. 1994 Jan 11;33(1):134-42. doi: 10.1021/bi00167a017.
Zotero 插件