Haddad Filho J, Cedenho A P, de Freitas V
Human Reproduction Sector, Escola Paulista de Medicina/Universidade Federal de São Paulo, Brazil.
Sao Paulo Med J. 1998 May-Jun;116(3):1734-7. doi: 10.1590/s1516-31801998000300008.
Endometrial maturation, important in the diagnosis of infertile couples, has been evaluated since 1950 using the Noyes criteria. Nevertheless, there is no consensus regarding the most suitable period of the luteal phase for performing the biopsy.
This study evaluated the correlation between the histological dating of two endometrial biopsies performed in the same menstrual cycle, on luteal phase days six and ten.
Prospective study.
Human Reproduction Division of the Federal University of São Paulo, referral center.
Twenty-five women complaining of infertility had their menstrual cycles monitored by ultrasound and LH plasma levels, to obtain evidence of ovulation.
Endometrial biopsies were performed on luteal phase days LH + 6 and LH + 10 (luteal phase day 1 = LH + 1 = the day that follows LH peak). Dating was done according to morphometric criteria, in which an endometrium sample is considered out of phase if the minimum maturation delay is one day. On day LH + 6, blood was drawn for plasma progesterone level determination.
All patients had an ovulatory cycle (mean LH peak: 47.4 U/L; mean follicular diameter on LH peak day: 18.9 mm; mean endometrial thickness on LH peak day: 10.3 mm; mean plasma progesterone level on day LH + 6: 14.4 ng/ml). 14 patients had both biopsies in phase; 5 patients had out of phase biopsies only on day LH + 6; 3 had out of phase biopsies only on day LH + 10 and 3 patients had out of phase biopsies on both days. McNemar's test showed no statistical difference between these data (p > 33.36%).
The correlation found between the endometrial datings suggests that biopsies performed on either of these two days are suitable for evaluation of endometrial maturation.
自1950年以来，一直使用诺伊斯标准评估子宫内膜成熟度，这对不孕夫妇的诊断很重要。然而，关于黄体期进行活检的最合适时间，目前尚无共识。
本研究评估了在同一个月经周期的黄体期第6天和第10天进行的两次子宫内膜活检的组织学分期之间的相关性。
前瞻性研究。
圣保罗联邦大学人类生殖科，转诊中心。
25名主诉不孕的女性通过超声和LH血浆水平监测其月经周期，以获取排卵证据。
在黄体期LH + 6天和LH + 10天（黄体期第1天 = LH + 1 = LH峰值后的第二天）进行子宫内膜活检。根据形态计量学标准进行分期，如果最小成熟延迟为一天，则认为子宫内膜样本不同期。在LH + 6天，抽取血液测定血浆孕酮水平。
所有患者均有排卵周期（平均LH峰值：47.4 U/L；LH峰值日平均卵泡直径：18.9 mm；LH峰值日平均子宫内膜厚度：10.3 mm；LH + 6日平均血浆孕酮水平：14.4 ng/ml）。14名患者的两次活检均同期；5名患者仅在LH + 6天活检不同期；3名患者仅在LH + 10天活检不同期；3名患者在两天的活检均不同期。McNemar检验显示这些数据之间无统计学差异（p > 33.36%）。
子宫内膜分期之间的相关性表明，在这两天中的任何一天进行活检都适合评估子宫内膜成熟度。
