Bachtiar E W, Cornain S, Siregar B, Raharjo T W
Department of Oral Biology, Faculty of Dentistry, University of Indonesia, Jakarta.
Asian Pac J Allergy Immunol. 1998 Jun-Sep;16(2-3):75-9.
The etiology of recurrent aphthous ulcers (RAU) has not been clearly defined. However, the results of several studies indicated the evidence of the role of immunological factors. The association between the regulator and effector component of the immune system in RAU needs clarifying by comparing major and minor type of RAU patients. The proportion of peripheral blood lymphocyte subsets were enumerated during active ulcer phase and analyzed in relation to ulcer types. Nineteen patients with RAU (12 minor type and 7 major type) and 8 healthy volunteers, of both sexes, aged 24-54 years old were tested. CD3+ (T cell), CD4+ (helper T cell), CD8+ (suppressor/cytotoxic T cell), CD19+ (B cell), and CD16+/CD56+ (NK cell) were determined by using appropriate monoclonal antibodies in double colored flow cytometry. The results showed that CD4+ was lower in RAU than control (P < 0.01). Comparing both types of RAU, it appeared that CD8+ was higher in the major type than the minor type (p < 0.01); CD4+/CD8+ ratio in the major type was lower than the minor type (P < 0.01). There was no difference in CD19+ and CD16+/CD56+ between any groups compared. The finding indicated that RAU was associated with abnormal proportions of CD4+ and CD8+ cells which was dependent on the severity of the lesion.
复发性阿弗他溃疡（RAU）的病因尚未明确界定。然而，多项研究结果表明了免疫因素作用的证据。RAU中免疫系统调节成分与效应成分之间的关联需要通过比较RAU患者的重型和轻型来阐明。在溃疡活动期对外周血淋巴细胞亚群比例进行计数，并根据溃疡类型进行分析。对19例RAU患者（12例轻型和7例重型）以及8名年龄在24至54岁之间的男女健康志愿者进行了检测。通过双色流式细胞术使用适当的单克隆抗体测定CD3 +（T细胞）、CD4 +（辅助性T细胞）、CD8 +（抑制性/细胞毒性T细胞）、CD19 +（B细胞）和CD16 + / CD56 +（NK细胞）。结果显示，RAU患者的CD4 +低于对照组（P < 0.01）。比较两种类型的RAU，重型患者的CD8 +高于轻型患者（p < 0.01）；重型患者的CD4 + / CD8 +比值低于轻型患者（P < 0.01）。所比较的任何组之间的CD19 +和CD16 + / CD56 +均无差异。该发现表明，RAU与CD4 +和CD8 +细胞比例异常有关，且这取决于病变的严重程度。
