Puoliväli J, Jäkälä P, Koivisto E, Riekkinen P
Department of Neuroscience and Neurology, University of Kuopio and Kuopio University Hospital, Finland.
Psychopharmacology (Berl). 1998 Dec;140(3):285-92. doi: 10.1007/s002130050769.
We investigated whether the local intrathalamic infusion of a muscarinic acetylcholine receptor agonist (oxotremorine) at either the reticular nucleus of thalamus (NRT) or the ventroposteromedial nucleus of thalamus (VPM) suppresses thalamocortically generated neocortical high-voltage spindles (HVSs). In addition, we studied whether the intracerebroventricular (ICV) infusion of a selective muscarinic M2 acetylcholine receptor antagonist (methoctramine) could block the suppression of HVSs induced by either systemic (IP) administration of an anticholinesterase drug [tetrahydroaminoacridine (THA)] or ICV infusion of oxotremorine in rats. Intrathalamic administration of oxotremorine at 3 and 15 microg in the NRT, and at 15 microg in the VPM suppressed HVSs. ICV oxotremorine at 30 and 100 microg and IP THA at 3 mg/kg decreased HVSs. ICV methoctramine at 100 microg increased HVSs and completely blocked the decrease in HVSs produced by oxotremorine 100 microg and THA 3 mg/kg. The results suggest that activation of muscarinic M2 acetylcholine receptors in thalamic nuclei (NRT and VPM) can suppress thalamocortical oscillations and that ICV or systemically administered drugs that activate either directly (oxotremorine and methoctramine) or indirectly (THA) the muscarinic M2 acetylcholine receptors may modulate neocortical HVSs via the thalamus.
我们研究了在丘脑网状核（NRT）或丘脑腹后内侧核（VPM）局部注射毒蕈碱型乙酰胆碱受体激动剂（氧化震颤素）是否会抑制丘脑皮质产生的新皮质高压纺锤波（HVSs）。此外，我们还研究了脑室内（ICV）注射选择性毒蕈碱M2型乙酰胆碱受体拮抗剂（甲溴东莨菪碱）是否能阻断抗胆碱酯酶药物[四氢氨基吖啶（THA）]全身（腹腔注射）给药或ICV注射氧化震颤素诱导的大鼠HVSs抑制。在NRT中注射3微克和15微克以及在VPM中注射15微克的氧化震颤素可抑制HVSs。ICV注射30微克和100微克的氧化震颤素以及腹腔注射3毫克/千克的THA可减少HVSs。ICV注射100微克的甲溴东莨菪碱可增加HVSs，并完全阻断100微克氧化震颤素和3毫克/千克THA引起的HVSs减少。结果表明，丘脑核（NRT和VPM）中毒蕈碱M2型乙酰胆碱受体的激活可抑制丘脑皮质振荡，并且ICV或全身给药的药物，无论是直接（氧化震颤素和甲溴东莨菪碱）还是间接（THA）激活毒蕈碱M2型乙酰胆碱受体，都可能通过丘脑调节新皮质HVSs。
