Pol A, Calvo M, Enrich C
Departament de Biologia Cellular, Institut d'Investigacions Biomèdiques August Pi i Sunyer, Facultat de Medicina, Universitat de Barcelona, Spain.
FEBS Lett. 1998 Dec 11;441(1):34-8. doi: 10.1016/s0014-5793(98)01517-8.
In this study we identify the molecules involved in the MAPK signal transduction pathway (Ras, Raf-1, Mek, Mek-P and MAPK) in highly purified endosomal fractions isolated from rat liver. Biochemical analysis shows that only the early-sorting endocytic compartment contains activated Raf-1 and Mek. Finally, the exogenous administration of EGF led to redistribution of Raf-1 from the caveolin-enriched plasma membrane into the endosomes.
在本研究中，我们在从大鼠肝脏分离出的高度纯化的内体组分中鉴定了参与丝裂原活化蛋白激酶（MAPK）信号转导途径（Ras、Raf-1、Mek、Mek-P和MAPK）的分子。生化分析表明，只有早期分选的内吞区室含有活化的Raf-1和Mek。最后，表皮生长因子（EGF）的外源给药导致Raf-1从富含小窝蛋白的质膜重新分布到内体中。
