Kawasaki Y, Fujii J, Miyazawa N, Hoshi A, Okado A, Tano Y, Taniguchi N
Department of Biochemistry, Osaka University Medical School, Suita, Japan.
FEBS Lett. 1998 Dec 11;441(1):116-20. doi: 10.1016/s0014-5793(98)01529-4.
The glycation reaction by fructose, as well as that by glucose, in control and diabetic rat lens was analyzed by using antibodies which specifically recognize adducts of lysine with fructose and with glucose. Levels of fructose adducts in diabetic rat lens were 2.5 times that of the control, and correlated with sorbitol levels. This was mainly due to enhanced glycation of beta- and gamma-crystallins by fructose under diabetic conditions. These data suggest that glycation by fructose may also play a role in cataract formation under conditions of diabetes and aging.
利用特异性识别赖氨酸与果糖及葡萄糖加合物的抗体，分析了对照大鼠和糖尿病大鼠晶状体中果糖以及葡萄糖引发的糖基化反应。糖尿病大鼠晶状体中果糖加合物的水平是对照大鼠的2.5倍，且与山梨醇水平相关。这主要是由于在糖尿病条件下，果糖使β-和γ-晶状体蛋白的糖基化增强。这些数据表明，在糖尿病和衰老情况下，果糖引发的糖基化反应可能也在白内障形成过程中发挥作用。
