Frøystad M K, Rode M, Berg T, Gjøen T
University of Oslo, Institute of Biology, Division of Molecular Cell Biology, Norway.
Dev Comp Immunol. 1998 Sep-Dec;22(5-6):533-49. doi: 10.1016/s0145-305x(98)00032-9.
In macrophages of higher vertebrates, Fc receptors and receptors for complement and other serum factors, are generally known to enhance the phagocytic process. In lower vertebrates like salmonid fishes, none of these or other phagocytic receptors have been thoroughly characterized. The purpose of this study was to elucidate to what extent these and other receptors are involved in the process of phagocytosis in rainbow trout (Oncorhynchus mykiss) head kidney macrophages. We used tosyl activated, paramagnetic dynabeads (2.8 microm in diameter), specifically coated with 125I labeled Atlantic salmon (Salmo salar) IgM or bovine serum albumin (BSA) as phagocytic probes. The effect of complement opsonization was also investigated by incubating the beads in serum. Our results indicate that neither the Fc- nor the complement-receptor(s) were important for phagocytosis of these beads. Our data support the idea that scavenger receptors are involved in phagocytosis in rainbow trout head kidney macrophages, as the use of a competitive scavenger receptor ligand extensively decreased degradation of the labeled protein coat on the beads.
在高等脊椎动物的巨噬细胞中，Fc受体、补体受体以及其他血清因子的受体通常被认为可增强吞噬过程。在像鲑科鱼类这样的低等脊椎动物中，这些或其他吞噬受体均未得到充分表征。本研究的目的是阐明这些受体及其他受体在虹鳟（Oncorhynchus mykiss）头肾巨噬细胞吞噬过程中的参与程度。我们使用经甲苯磺酰活化的顺磁性磁珠（直径2.8微米），其表面特异性包被有125I标记的大西洋鲑（Salmo salar）IgM或牛血清白蛋白（BSA）作为吞噬探针。还通过将磁珠在血清中孵育来研究补体调理作用的影响。我们的结果表明，Fc受体和补体受体对这些磁珠的吞噬作用均不重要。我们的数据支持这样一种观点，即清道夫受体参与虹鳟头肾巨噬细胞的吞噬作用，因为使用竞争性清道夫受体配体可大幅降低磁珠上标记蛋白涂层的降解。
