Files J G, Gray J L, Do L T, Foley W P, Gabe J D, Nestaas E, Pungor E
Berlex Laboratories, Inc., Richmond, CA 94804-0099, USA.
J Interferon Cytokine Res. 1998 Dec;18(12):1019-24. doi: 10.1089/jir.1998.18.1019.
We describe a novel MxA gene-induction assay for type I interferons (IFN-alpha and IFN-beta) based on the specific induction of the MxA gene in cultured human cells. Accumulated intracellular MxA protein is determined by immunologic measurement by a rapid method using commercially available materials. IFN activity can be measured accurately over a concentration range of 0.1-30 IU/ml. In contrast, type II IFN and other cytokines are not significantly detected. The MxA-induction assay has advantages in terms of specificity, reliability, and sensitivity over other methods for assaying type I IFN. It has also been adapted and validated for measuring the titers of anti-IFN-beta neutralizing antibodies in human sera.
我们描述了一种基于培养的人类细胞中Mx A基因的特异性诱导来检测I型干扰素（IFN-α和IFN-β）的新型Mx A基因诱导检测方法。通过使用市售材料的快速方法进行免疫测定来确定细胞内积累的Mx A蛋白。在0.1-30 IU/ml的浓度范围内可以准确测量IFN活性。相比之下，未显著检测到II型IFN和其他细胞因子。与其他检测I型IFN的方法相比，Mx A诱导检测方法在特异性、可靠性和灵敏度方面具有优势。它也已被改编并验证用于测量人血清中抗IFN-β中和抗体的滴度。
