Bottecchia D, Vermylen J
Br J Haematol. 1976 Oct;34(2):303-11. doi: 10.1111/j.1365-2141.1976.tb00200.x.
Bovine factor VIII is a potent inducer of aggregation of human platelets. Upon gel filtration of five-thousand-fold purified material in 0.5 M CaCl2, bovine factor VIII is separated into high and low molecular weight components; the former contains both a 'carrier protein' and platelet aggregating activity, the latter the procoagulant activity (low molecular weight factor VII, LMW-FVIII). Upon removal of Ca2+ ions, LMW-F VIII recombines with the 'carrier protein'. LMW-F VIII modifies aggregation by 'carrier protein', but not aggregation by undissociated bovine factor VII, adenosine-5'-diphosphate or adrenaline. This finding indicates that the platelet aggregating activity in indeed a property of the 'carrier protein'.
牛因子VIII是人类血小板聚集的强效诱导剂。在0.5M氯化钙中对经过五千倍纯化的物质进行凝胶过滤时，牛因子VIII被分离为高分子量和低分子量组分；前者同时含有一种“载体蛋白”和血小板聚集活性，后者含有促凝血活性（低分子量因子VIII，LMW-FVIII）。去除钙离子后，LMW-FVIII与“载体蛋白”重新结合。LMW-FVIII通过“载体蛋白”改变聚集，但不改变未解离的牛因子VIII、腺苷-5'-二磷酸或肾上腺素所引起的聚集。这一发现表明血小板聚集活性确实是“载体蛋白”的一种特性。
