Gregorc A, Bowen I D
Veterinary Faculty of the University of Ljubljana, Gerbiceva 60, Ljubljana, 1000, Slovenia.
Cell Biol Int. 1998;22(2):137-44. doi: 10.1006/cbir.1998.0232.
Morphological, histochemical and cytochemical changes were examined in honeybee larvae after infection with the bacterium Bacillus larvae. The results indicate cell necrosis in the midgut epithelium accompanied by increasing cell vacuolization and nuclear pyknosis following per os inoculation with B. larvae. Many autolysosomes were positive for acid phosphatase. Non-vacuolar acid phosphatase activity was also found in lysed cell compartments. No such activity was found in regenerative epithelial cells. Degradation of haemocytes, salivary glands and other tissues was also observed. Histochemical analyses after per cutaneous inoculation with B. larvae of three- and five-day-old honeybee larvae show intense non-vacuolar acid phosphatase activity followed by disintegration of infected salivary glands, epithelial cell cytoplasm and haemocytes.
对感染幼虫芽孢杆菌的蜜蜂幼虫进行了形态学、组织化学和细胞化学变化研究。结果表明，经口接种幼虫芽孢杆菌后，中肠上皮出现细胞坏死，同时细胞空泡化增加和核固缩。许多自噬溶酶体酸性磷酸酶呈阳性。在裂解的细胞区室中也发现了非空泡酸性磷酸酶活性。在再生上皮细胞中未发现此类活性。还观察到血细胞、唾液腺和其他组织的降解。对三日龄和五日龄蜜蜂幼虫经皮接种幼虫芽孢杆菌后的组织化学分析显示，感染唾液腺、上皮细胞胞质和血细胞出现强烈的非空泡酸性磷酸酶活性，随后发生崩解。
