Saitoh M, ten Dijke P, Miyazono K, Ichijo H
Department of Biochemistry, Cancer Institute, Japanese Foundation for Cancer Research and Research for the Future Program, Tokyo.
Biochem Biophys Res Commun. 1998 Dec 18;253(2):470-6. doi: 10.1006/bbrc.1998.9784.
The human cDNA encoding a novel protein serine/threonine kinase most closely related to p70 S6 kinase was isolated from the human erythroleukemia cDNA library and termed p70(S6Kbeta). p70(S6Kbeta) has 67% amino acid identity in overall sequence with human p70(S6K), and the potential phosphorylation sites of p70(S6K) are conserved in p70(S6Kbeta). Northern blot analysis identified two major transcripts of p70(S6Kbeta) that are ubiquitously expressed in human adult tissues. Similar to p70(S6K), p70(S6Kbeta) was activated by serum stimulation, and the serum-induced activation was inhibited by wortmannin and rapamycin. These findings suggest that p70(S6Kbeta) is an isoform of p70(S6K) with similar regulatory mechanisms.
从人红白血病cDNA文库中分离出编码一种与p70 S6激酶关系最为密切的新型蛋白丝氨酸/苏氨酸激酶的人cDNA，并将其命名为p70(S6Kbeta)。p70(S6Kbeta)在整体序列上与人类p70(S6K)有67%的氨基酸同一性，且p70(S6K)的潜在磷酸化位点在p70(S6Kbeta)中保守。Northern印迹分析鉴定出p70(S6Kbeta)的两种主要转录本，它们在人类成人组织中普遍表达。与p70(S6K)相似，p70(S6Kbeta)被血清刺激激活，且渥曼青霉素和雷帕霉素可抑制血清诱导的激活。这些发现表明p70(S6Kbeta)是p70(S6K)的一种同工型，具有相似的调节机制。
