Chiles R E, Reisen W K
Arbovirus Research Unit, School of Veterinary Medicine, University of California, Davis 95616, USA.
J Vector Ecol. 1998 Dec;23(2):123-35.
A new indirect enzyme immunoassay (EIA) was developed to screen wild bird sera for antibodies against western equine encephalomyelitis (WEE) and St. Louis encephalitis (SLE) viruses. The detector antibody was made by immunizing rabbits with serum proteins pooled from single species representatives of four bird orders and was conjugated with horseradish peroxidase to allow visualization with the ABTS substrate in an EIA plate reader set at 405 nm. The detector antibody recognized a wide range of bird species and was more accurate, sensitive, and specific than a hemaglutination inhibition test when compared to a plaque reduction neutralization test (PRNT). EIA positive sera frequently could not be confirmed by PRNT; however, practically all sera positive by PRNT also were positive by EIA. The new EIA has been incorporated into our field research program and has been used to economically screen over 10,000 wild bird sera from 124 species for antibodies against WEE and SLE.
开发了一种新的间接酶免疫测定法（EIA），用于筛选野生鸟类血清中针对西部马脑炎（WEE）和圣路易斯脑炎（SLE）病毒的抗体。检测抗体是通过用从四个鸟类目单一种类代表中收集的血清蛋白免疫兔子制备的，并与辣根过氧化物酶结合，以便在设置为405nm的酶免疫测定板读数器中用ABTS底物进行可视化。该检测抗体识别多种鸟类，与蚀斑减少中和试验（PRNT）相比，比血凝抑制试验更准确、灵敏和特异。EIA阳性血清通常不能通过PRNT确认；然而，实际上所有PRNT阳性的血清EIA也呈阳性。这种新的EIA已被纳入我们的野外研究计划，并已用于经济地筛选来自124个物种的10000多份野生鸟类血清中针对WEE和SLE的抗体。
