Borel E, Mayençon M, Kaiser K, Picot S, Peyron F
Laboratoire de Parasitologie-Mycologie Médicale et Pathologie Exotique, Université Claude Bernard Lyon I.
Parasite. 1998 Dec;5(4):371-3. doi: 10.1051/parasite/1998054371.
In order to easily assess growth and destruction of Toxoplasma gondii in vitro, this report describes two double staining assays that both visualize live and dead organisms: acridine orange--ethidium bromide (AO-EB) and bisbenzimide (Hoechst 33258)--propidium iodide (B-PI). EB and PI were chosen for dead organisms staining while AO and B stain viable organisms. Thus, both double staining assays seem more informative than Giemsa staining or indirect immunofluorescence. They offer methods to study internal structure of the parasite as well as information on host-parasite relationships. Moreover, detection in culture are sensitive, easier, and less time consuming than previous methods. So, they should to be useful in strains behaviour analysis.
为了便于在体外评估刚地弓形虫的生长和破坏情况，本报告描述了两种能同时观察活生物体和死生物体的双重染色测定法：吖啶橙-溴化乙锭（AO-EB）和双苯甲酰亚胺（Hoechst 33258）-碘化丙啶（B-PI）。选择EB和PI用于死生物体染色，而AO和B用于活生物体染色。因此，这两种双重染色测定法似乎比吉姆萨染色或间接免疫荧光更具信息性。它们提供了研究寄生虫内部结构以及宿主-寄生虫关系信息的方法。此外，与以前的方法相比，在培养物中的检测更灵敏、更容易且耗时更少。所以，它们应该对菌株行为分析有用。
