Yoshida K, Nakamura W, Hirano K, Yuasa H, Tsukamoto T, Tatematsu M
Laboratory of Pathology, Aichi Cancer Center Research Institute, Nagoya, Japan.
J Cancer Res Clin Oncol. 1998;124(12):677-82. doi: 10.1007/s004320050231.
In this study the small-intestine phenotype in rat colonic tumors was investigated in terms of sucrase and intestinal-type alkaline phosphatase (I-ALP) activity. F344 rats were given intraperitoneal injections of methylazoxymethanol acetate at a dose level of 25 mg/kg body weight once a week for 8 weeks and were killed 40 weeks after the first injection. Sucrase and I-ALP activities in proximal and distal colon adenocarcinomas were significantly higher than those in the normal colon epithelium. In the jejunum, by contrast, normal tissue had significantly higher levels than tumors. Immunohistochemical staining of I-ALP was also strong in striated cell borders of colon adenocarcinoma cells. These data suggest that, whereas absorptive cells of the small intestine lose their own traits with tumor development, colonocytes acquire phenotypic features of the small intestine. Intestinal enzymes associated with the striated-cell border, such as sucrase and I-ALP, may be useful markers for malignant phenotypic expression in colonocytes.
在本研究中，从蔗糖酶和肠型碱性磷酸酶（I-ALP）活性方面对大鼠结肠肿瘤中的小肠表型进行了研究。给F344大鼠腹腔注射剂量为25 mg/kg体重的乙酸甲基氧化偶氮甲醇，每周一次，共8周，并在首次注射后40周处死。近端和远端结肠腺癌中的蔗糖酶和I-ALP活性显著高于正常结肠上皮中的活性。相比之下，在空肠中，正常组织的水平显著高于肿瘤组织。I-ALP的免疫组织化学染色在结肠腺癌细胞的纹状细胞边界也很强。这些数据表明，虽然小肠的吸收细胞随着肿瘤发展失去了自身特征，但结肠细胞获得了小肠的表型特征。与纹状细胞边界相关的肠道酶，如蔗糖酶和I-ALP，可能是结肠细胞恶性表型表达的有用标志物。
