Marty N, Pasquier C, Dournes J L, Chemin K, Chavagnat F, Guinand M, Chabanon G, Pipy B, Montrozier H
Laboratoire de Microbiologie, Université Paul Sabatier, Toulouse, France.
J Med Microbiol. 1998 Feb;47(2):129-34. doi: 10.1099/00222615-47-2-129.
This study evaluated, in vitro, the role of different Pseudomonas aeruginosa exopolysaccharides (EPS) in mediating adherence to human respiratory epithelial cells. Two mucoid and non-mucoid isogenic pairs of P aeruginosa strains isolated from patients with cystic fibrosis (CF) and bronchiectasis were used. Adherence was tested with human tracheal epithelial cell lines from CF and normal fetuses. The CF cells bound significantly more bacteria than the normal cells. The strain from the bronchiectasis patient was significantly more adherent than that from the CF patient and this difference was consistently most marked with the non-mucoid variant and with normal epithelial cells. The differing behaviour of mucoid CF and non-mucoid bronchiectasis strains reflected the chemical composition of their EPS: mainly alginate in the former and neutral polysaccharides in the latter. Additive inhibition experiments with chemically characterised EPS indicated that neutral polysaccharides associated with alginate may act as ligands for the adherence of P. aeruginosa to CF epithelial cells.
本研究在体外评估了不同铜绿假单胞菌胞外多糖（EPS）在介导对人呼吸道上皮细胞黏附中的作用。使用了从囊性纤维化（CF）患者和支气管扩张患者中分离出的两对黏液型和非黏液型同基因铜绿假单胞菌菌株。用来自CF患者和正常胎儿的人气管上皮细胞系测试黏附情况。CF细胞结合的细菌明显多于正常细胞。支气管扩张患者的菌株比CF患者的菌株黏附性明显更强，这种差异在非黏液型变体和正常上皮细胞中始终最为显著。黏液型CF菌株和非黏液型支气管扩张菌株的不同行为反映了它们EPS的化学组成：前者主要是藻酸盐，后者是中性多糖。用化学特征明确的EPS进行的加性抑制实验表明，与藻酸盐相关的中性多糖可能作为铜绿假单胞菌黏附CF上皮细胞的配体。
