Rajashekara G, Koeuth T, Nevile S, Back A, Nagaraja K V, Lupski J R, Kapur V
Department of Veterinary PathoBiology, University of Minnesota, St Paul 55108, USA.
J Med Microbiol. 1998 Jun;47(6):489-97. doi: 10.1099/00222615-47-6-489.
The presence of a Salmonella serotype Enteritidis repeat element (SERE) located within the upstream regulatory region of the sefABCD operon encoding fimbrial proteins is reported. DNA dot-blot hybridisation analyses and computerised searches of genetic databases indicate that SERE is well conserved and widely distributed throughout the bacterial and archaeal kingdoms. A SERE-based polymerase chain reaction (SERE-PCR) assay was developed to fingerprint 54 isolates of Enteritidis representing nine distinct phage types and 54 isolates of other Salmonella serotypes. SERE-PCR identified five distinct fingerprint profiles among the 54 Enteritidis isolates; no correlation between phage types and SERE-PCR fingerprint patterns was noticed. SERE-PCR was reproducible, rapid and easy to perform. The results of this investigation suggest that the limited heterogeneity of SERE-PCR fingerprint patterns can be utilised to develop serotype- and serogroup-specific fingerprint patterns for isolates of Enteritidis.
据报道，在编码菌毛蛋白的sefABCD操纵子的上游调控区域内存在肠炎沙门氏菌重复元件（SERE）。DNA斑点杂交分析和遗传数据库的计算机搜索表明，SERE在细菌和古菌界中保守性良好且分布广泛。开发了一种基于SERE的聚合酶链反应（SERE-PCR）检测方法，用于对代表9种不同噬菌体类型的54株肠炎沙门氏菌分离株和54株其他沙门氏菌血清型分离株进行指纹图谱分析。SERE-PCR在54株肠炎沙门氏菌分离株中鉴定出5种不同的指纹图谱；未发现噬菌体类型与SERE-PCR指纹图谱之间存在相关性。SERE-PCR具有可重复性、快速且易于操作。本研究结果表明，SERE-PCR指纹图谱的有限异质性可用于为肠炎沙门氏菌分离株开发血清型和血清群特异性指纹图谱。
