Fadl A A, Nguyen A V, Khan M I
Department of Pathobiology, University of Connecticut, Storrs 06269-3089, USA.
J Clin Microbiol. 1995 Apr;33(4):987-9. doi: 10.1128/jcm.33.4.987-989.1995.
An arbitrarily primed PCR (AP-PCR) was developed to analyze the genomic DNAs of Salmonella enteritidis isolates from human outbreaks and from avian sources. The AP-PCR generated seven distinct randomly amplified DNA patterns among the S. enteritidis isolates studied. Differences in the DNA patterns among isolates of S. enteritidis phage types 13a and 8 as well as among S. enteritidis phage type 14b were observed. The AP-PCR analysis can be used to determine the differences among isolates within the same phage types and may be useful for tracing back the source of S. enteritidis outbreaks in humans more precisely.
开发了一种任意引物PCR（AP-PCR）方法，用于分析从人类疫情和禽类来源分离的肠炎沙门氏菌的基因组DNA。在所研究的肠炎沙门氏菌分离株中，AP-PCR产生了七种不同的随机扩增DNA图谱。观察到肠炎沙门氏菌噬菌体类型13a和8的分离株之间以及肠炎沙门氏菌噬菌体类型14b的分离株之间DNA图谱的差异。AP-PCR分析可用于确定同一噬菌体类型内分离株之间的差异，可能有助于更精确地追溯人类肠炎沙门氏菌疫情的源头。
