一种来自玉米幼苗的用于非色氨酸依赖型吲哚-3-乙酸生物合成的体外系统。
An in vitro system from maize seedlings for tryptophan-independent indole-3-acetic acid biosynthesis.
作者信息
Ostin A, Ilic N, Cohen JD
机构信息
Department of Forest Genetics and Plant Physiology, The Swedish University of Agricultural Sciences, 90183 Umea, Sweden (A.O.).
出版信息
Plant Physiol. 1999 Jan;119(1):173-8. doi: 10.1104/pp.119.1.173.
Abstract
The enzymatic synthesis of indole-3-acetic acid (IAA) from indole by an in vitro preparation from maize (Zea mays L.) that does not use tryptophan (Trp) as an intermediate is described. Light-grown seedlings of normal maize and the maize mutant orange pericarp were shown to contain the necessary enzymes to convert [14C]indole to IAA. The reaction was not inhibited by unlabeled Trp and neither [14C]Trp nor [14C]serine substituted for [14C]indole in this in vitro system. The reaction had a pH optimum greater than 8.0, required a reducing environment, and had an oxidation potential near that of ascorbate. The results obtained with this in vitro enzyme preparation provide strong, additional evidence for the presence of a Trp-independent IAA biosynthesis pathway in plants.
摘要
本文描述了一种来自玉米（Zea mays L.）的体外制剂，该制剂能在不使用色氨酸（Trp）作为中间体的情况下，将吲哚酶促合成吲哚-3-乙酸（IAA）。研究表明，正常玉米和玉米突变体橙色果皮的光照生长幼苗含有将[14C]吲哚转化为IAA所需的酶。在该体外系统中，未标记的Trp不会抑制该反应，且[14C]Trp和[14C]丝氨酸均不能替代[14C]吲哚。该反应的最适pH大于8.0，需要还原环境，且氧化电位接近抗坏血酸盐。用这种体外酶制剂获得的结果为植物中存在不依赖Trp的IAA生物合成途径提供了有力的额外证据。
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本文引用的文献
1
Indole-3-Acetic Acid Biosynthesis in the Mutant Maize orange pericarp, a Tryptophan Auxotroph.突变型玉米橙色果皮中吲哚-3-乙酸的生物合成，色氨酸营养缺陷型。
Science. 1991 Nov 15;254(5034):998-1000. doi: 10.1126/science.254.5034.998.
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