以氧化氘为示踪剂对玉米中吲哚-3-乙酸代谢的分析
Analysis of Indole-3-acetic Acid Metabolism in Zea mays Using Deuterium Oxide as a Tracer.
作者信息
Pengelly W L, Bandurski R S
机构信息
Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824.
出版信息
Plant Physiol. 1983 Oct;73(2):445-9. doi: 10.1104/pp.73.2.445.
Abstract
A method using deuterium oxide (D(2)O) as a tracer was used to study indole-3-acetic acid (IAA) metabolism in Zea mays seedlings. Seeds were imbibed and grown for 4 days in 30% D(2)O in the dark. IAA was then isolated from roots and shoots and analyzed for deuterium content by mass spectrometry. We found that a significant portion of the IAA isolated from plants had incorporated deuterium at nonexchangeable sites of the indole ring. This indicates that some of the IAA in the germinating seedling is made via de novo indole synthesis. Moreover, we found that the deuterium content of IAA was 2.6 times greater in shoots than in roots. These results indicate that at least some of the IAA in roots and shoots came from different biosynthetic pathways. It appears that the fraction of IAA produced via de novo indole synthesis is greater in shoots than in roots.
摘要
采用以重水（D₂O）作为示踪剂的方法来研究玉米幼苗中吲哚 - 3 - 乙酸（IAA）的代谢。种子在黑暗中于30%的重水中浸泡并生长4天。然后从根和芽中分离出IAA，并通过质谱分析其氘含量。我们发现，从植物中分离出的IAA的很大一部分在吲哚环的非交换位点掺入了氘。这表明发芽幼苗中的一些IAA是通过从头合成吲哚产生的。此外，我们发现芽中IAA的氘含量是根中的2.6倍。这些结果表明，根和芽中至少一些IAA来自不同的生物合成途径。似乎通过从头合成吲哚产生的IAA部分在芽中比在根中更大。
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