Garland J T, Buchanan F
J Clin Endocrinol Metab. 1976 Oct;43(4):842-6. doi: 10.1210/jcem-43-4-842.
The stimulation of isolated chicken embryo chondrocytes was studied by measuring the incorporation of [3H]uridine and [3H]leucine into cold trichloroacetic acid precipitable material after exposure of the chondrocytes to serum. The doseresponse relationships for the incorporation of uridine and leucine were similar to that of thymidine previously demonstrated. Exposure of the cells to serum-containing buffer for 15 min sufficed both for the stimulation of incorporation into the cells and for the depletion of 28% of the stimulating activity from the medium. Stimulation persisted for at least 17 h after removal of the serum. Studies where actinomycin D was added to inhibit RNA synthesis suggested that prior RNA synthesis was required for most of the stimulation of protein synthesis by serum factors.
通过测定软骨细胞暴露于血清后，[3H]尿苷和[3H]亮氨酸掺入冷三氯乙酸可沉淀物质中的情况，研究了分离的鸡胚软骨细胞的刺激作用。尿苷和亮氨酸掺入的剂量反应关系与先前证明的胸苷相似。将细胞暴露于含血清缓冲液15分钟，足以刺激其掺入细胞，并使培养基中28%的刺激活性耗尽。去除血清后，刺激作用持续至少17小时。添加放线菌素D抑制RNA合成的研究表明，血清因子对蛋白质合成的大部分刺激作用需要先前的RNA合成。
