Kanazawa A, Tozuka A, Akimoto S, Abe J, Shimamoto Y
Laboratory of Plant Genetics and Evolution, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
Genes Genet Syst. 1998 Aug;73(4):255-61. doi: 10.1266/ggs.73.255.
Restriction fragment length polymorphisms (RFLPs) of mitochondrial DNA (mtDNA) of wild and cultivated soybeans were analyzed to study their phylogenetic relationships. The observed number of differences in hybridization profiles greatly varied (1-17 patterns) with both the mtDNA probes and the restriction enzymes that were used. A cladistic analysis was conducted based on the RFLP data. In the parsimonious tree, four distinct groups appeared among 20 accessions of the subgenus Soja representing 20 mitochondrial genome types. Common features with regard to geographic distributions in natural populations in East Asia were observed among the mitochondrial genome types of wild soybean that belonged to the same group: one clade consisted of genome types IIg and VIIg that are detected with very low frequencies; another clade consisted of genome types Ic, Id, Ie, and Ik whose distributions are highly biased mainly in Japan. The genome types that are widely distributed in East Asia such as IVa, IVb, and Va were not grouped into the same clade. The mitochondrial genome types IIIb, IVb, and IVc, in which two different chloroplast genome types exist, belonged to the same clade. Possible changes in mitochondrial genomes during the expansion of the distribution of wild soybeans in East Asia were discussed.
分析野生和栽培大豆线粒体DNA（mtDNA）的限制性片段长度多态性（RFLP），以研究它们的系统发育关系。观察到的杂交图谱差异数量因所使用的mtDNA探针和限制性内切酶而有很大差异（1 - 17种模式）。基于RFLP数据进行了分支分析。在简约树中，20个大豆亚属材料代表20种线粒体基因组类型，分为四个不同的组。属于同一组的野生大豆线粒体基因组类型在东亚自然种群的地理分布上具有共同特征：一个分支由频率极低的IIg和VIIg基因组类型组成；另一个分支由Ic、Id、Ie和Ik基因组类型组成，其分布主要高度偏向于日本。在东亚广泛分布的基因组类型，如IVa、IVb和Va，并未归为同一分支。存在两种不同叶绿体基因组类型的线粒体基因组类型IIIb、IVb和IVc属于同一分支。讨论了东亚野生大豆分布扩展过程中线粒体基因组可能发生的变化。
