Powell W, Morgante M, Doyle J J, McNicol J W, Tingey S V, Rafalski A J
Department of Cell and Molecular Genetics, Scottish Crop Research Institute, Invergowrie, Dundee, Scotland.
Genetics. 1996 Oct;144(2):793-803. doi: 10.1093/genetics/144.2.793.
A combination of nuclear and chloroplast simple sequence repeats (SSRs) have been used to investigate the levels and pattern of variability detected in Glycine max and G. soja genotypes. Based on the analysis of 700 soybean genotypes with 115 restriction fragment length polymorphism (RFLP) probes, 12 accessions were identified that represent 92% of the allelic variability detected in this genepool. These 12 core genotypes together with a sample of G. max and G. soja accessions were evaluated with 11 nuclear SSRs that detected 129 alleles. Compared with the other G. max and G. soja genotypes sampled, the core genotypes represent 40% of the allelic variability detected with SSRs. Despite the multi-allelic nature of soybean SSRs, dendrograms representing phenetic relationships between accessions clustered according to their subspecies origin. In addition to biparentally inherited nuclear SSRs, two uniparentally (maternally) transmitted chloroplast SSRs were also studied. A total of seven haplotypes were identified, and diversity indices of 0.405 +/- 0.088 and 0.159 +/- 0.071 were obtained for the two chloroplast SSRs. The availability of polymorphic SSR loci in the chloroplast genome provides new opportunities to investigate cytonuclear interactions in plants.
已使用核简单序列重复（SSR）和叶绿体SSR的组合来研究在大豆（Glycine max）和野生大豆（G. soja）基因型中检测到的变异水平和模式。基于对700个大豆基因型使用115个限制性片段长度多态性（RFLP）探针的分析，鉴定出了12个种质，它们代表了在该基因库中检测到的92%的等位基因变异。用11个核SSR对这12个核心基因型以及大豆和野生大豆种质的一个样本进行了评估，共检测到129个等位基因。与所采样的其他大豆和野生大豆基因型相比，核心基因型代表了用SSR检测到的等位基因变异的40%。尽管大豆SSR具有多等位基因性质，但代表种质间表型关系的聚类图是根据它们的亚种起源聚类的。除了双亲遗传的核SSR外，还研究了两个单亲（母系）遗传的叶绿体SSR。总共鉴定出7种单倍型，两个叶绿体SSR的多样性指数分别为0.405±0.088和0.159±0.071。叶绿体基因组中多态SSR位点的可用性为研究植物中的细胞核与细胞质相互作用提供了新机会。
