Kassim O, Gibertson D E
J Parasitol. 1976 Oct;62(5):715-20.
Eggs of Schistosoma mansoni were obtained from livers of mice and hatching was observed under varying conditions of light and ionic composition of the medium. Hatching occurred equally well in light and in darkness. Eggs also hatched readily in 1- to 50-m OsM solutions of urea, sucrose, sodium chloride, and glycerol, but hatching was inhibited at higher concentrations unless the eggs were left in solutions for long periods of time. Hatching readily occurred in deionized water, but the emerged miracidia did not swim longer than 5 to 10 min unless Na+ was added. Histochemistry of the egg showed DNA-positive egg granules and a polysaccharide-positive vacuole matrix. Acid mucopolysaccharides were stained in the vacuolar matrix and in the anterior sac of the miracidium. Longitudinal alignment of constituents of the egg shell is suggested by the predominance of longitudinal rents in the shell at hatching. A mechanism of hatching involving an osmotic stimulus is proposed.
曼氏血吸虫卵取自小鼠肝脏，并在不同光照条件和培养基离子组成下观察孵化情况。在光照和黑暗条件下孵化效果相同。卵在1至50毫渗摩尔的尿素、蔗糖、氯化钠和甘油溶液中也能很容易地孵化，但在较高浓度下孵化受到抑制，除非将卵长时间留在溶液中。卵在去离子水中很容易孵化，但除非添加Na+，孵出的毛蚴游动时间不超过5至10分钟。卵的组织化学显示有DNA阳性的卵颗粒和多糖阳性的液泡基质。酸性粘多糖在液泡基质和毛蚴的前囊中被染色。孵化时卵壳纵向裂缝占优势，提示卵壳成分呈纵向排列。提出了一种涉及渗透刺激的孵化机制。
