Sung C K, Dresden M H
Am J Trop Med Hyg. 1984 Nov;33(6):1178-81. doi: 10.4269/ajtmh.1984.33.1178.
The viability of Schistosoma mansoni miracidia, either hatched or unhatched, has been assessed by a staining technique using Congo red, a pH-sensitive dye (pH 3-5), under the light microscope. Hatched, free-swimming miracidia and live miracidia inside egg shells were stained light blue whereas dying miracidia altered the dye color from blue to orange and eventually to red. Immature eggs containing undeveloped miracidia were not stained by Congo red. This staining technique is simple, rapid and can be used as a method to assess both the viability of S. mansoni miracidia and the hatching process.
利用刚果红（一种对pH敏感的染料，pH值为3 - 5）染色技术，在光学显微镜下评估曼氏血吸虫毛蚴（已孵化或未孵化）的活力。已孵化的、自由游动的毛蚴以及卵壳内的活毛蚴被染成浅蓝色，而濒死的毛蚴会使染料颜色从蓝色变为橙色，最终变为红色。含有未发育毛蚴的未成熟卵不会被刚果红染色。这种染色技术简单、快速，可作为评估曼氏血吸虫毛蚴活力和孵化过程的一种方法。
