Bolzán A D, Bianchi N O, Bianchi M S
Instituto Multidisciplinario de Biología Celular (IMBICE), La Plata, Argentina.
Environ Mol Mutagen. 1998;32(4):331-5. doi: 10.1002/(sici)1098-2280(1998)32:4<331::aid-em6>3.0.co;2-0.
We assessed the chromosomal response of insect (mosquito, Aedes albopictus) and mammalian (Chinese hamster ovary, CHO) cells to streptonigrin (SN). Both types of cells were pulse-treated for 20 min with increasing doses of SN and the frequency of chromosome aberrations and sister chromatid exchanges (SCEs) for each SN dose was determined. Our results show that the SN doses inducing remarkable chromosome damage (expressed as frequency of aberrations per cell and per chromosome) in CHO cells fail to produce a significant increase of aberrations in mosquito chromosomes. Moreover, CHO cells exhibited a dose-dependent increase in SCEs which was not observed in mosquito cells. Our results show that while mammalian cells are very sensitive, insect cells are very resistant to SN at the chromosome level. It is possible that variations in the chromatin fibril structure and in the intracellular antioxidant pool may be responsible for the differential response of insect and mammalian chromosomes to SN.
我们评估了昆虫（蚊子，白纹伊蚊）和哺乳动物（中国仓鼠卵巢细胞，CHO）细胞对链黑菌素（SN）的染色体反应。两种类型的细胞均用递增剂量的SN脉冲处理20分钟，并确定每个SN剂量下的染色体畸变频率和姐妹染色单体交换（SCE）频率。我们的结果表明，在CHO细胞中诱导显著染色体损伤（以每个细胞和每条染色体的畸变频率表示）的SN剂量未能使蚊子染色体的畸变显著增加。此外，CHO细胞中SCE呈剂量依赖性增加，而在蚊子细胞中未观察到这种情况。我们的结果表明，虽然哺乳动物细胞非常敏感，但昆虫细胞在染色体水平上对SN具有很强的抗性。染色质纤维结构和细胞内抗氧化剂库的差异可能是昆虫和哺乳动物染色体对SN产生不同反应的原因。
