Bolzán A D, González M C, Bianchi M S
Instituto Multidisciplinario de Biología Celular (IMBICE), C.C. 403, 1900, La Plata, Argentina.
Mutat Res. 2000 Feb 14;447(2):221-6. doi: 10.1016/s0027-5107(99)00210-9.
The effect of the metal chelating agent 1,10-Phenanthroline (PNT) on the streptozotocin (STZ)-induced chromosomal aberrations (CAs) and sister-chromatid exchanges (SCEs) in Chinese hamster ovary (CHO) and mosquito (Aedes albopictus) cells was investigated. Treatment of CHO and mosquito cells with STZ produced a significant and dose-response increase in the yield of CAs as well as SCEs (p<0.05). The addition of PNT prevented the induction of CAs by STZ in both types of cells, causing a significant decrease in the frequency of STZ-induced CAs (46.5-72.5%) (p<0.05). This fact indicates that intracellular transition metals are implicated in STZ-induced CAs and that the Fenton reaction (Fe(2+)+H(2)O(2)-->OH degrees +OH(-)+ Fe(3+)) is partly responsible for the production of CAs by this compound. On the other hand, the addition of PNT to CHO and mosquito cell cultures did not prevent the induction of SCEs by STZ. Therefore, it is valid to assume that the induction of CAs and SCEs by STZ occurs by different mechanisms.
研究了金属螯合剂1,10 - 菲咯啉（PNT）对链脲佐菌素（STZ）诱导的中国仓鼠卵巢（CHO）细胞和蚊子（白纹伊蚊）细胞染色体畸变（CAs）及姐妹染色单体交换（SCEs）的影响。用STZ处理CHO细胞和蚊子细胞后，CAs和SCEs的产率显著增加且呈剂量反应关系（p<0.05）。添加PNT可防止STZ在两种细胞类型中诱导CAs，使STZ诱导的CAs频率显著降低（46.5 - 72.5%）（p<0.05）。这一事实表明细胞内过渡金属与STZ诱导的CAs有关，且芬顿反应（Fe(2+)+H(2)O(2)-->OH•+OH(-)+ Fe(3+)）部分导致了该化合物诱导CAs的产生。另一方面，向CHO细胞和蚊子细胞培养物中添加PNT并不能阻止STZ诱导SCEs。因此，可以合理推测STZ诱导CAs和SCEs的机制不同。
