Herrera J B, Bartel B, Wilson W K, Matsuda S P
Department of Chemistry, Rice University, Houston, TX 77005, USA.
Phytochemistry. 1998 Dec;49(7):1905-11. doi: 10.1016/s0031-9422(98)00366-5.
A 2274 bp Arabidopsis thaliana cDNA was isolated that encodes a protein 57% identical to cycloartenol synthase from the same organism. The expressed recombinant protein encodes lupeol synthase, which converts oxidosqualene to the triterpene lupeol as the major product. Lupeol synthase is a multifunctional enzyme that forms other triterpene alcohols, including beta-amyrin, as minor products. Sequence analysis suggests that lupeol synthase diverged from cycloartenol synthase after plants diverged from fungi and animals. This evolutionary order is the reason that fungi and animals do not make lupeol.
分离出了一段2274 bp的拟南芥cDNA，它编码一种与来自同一生物体的环阿屯醇合酶有57%同一性的蛋白质。表达的重组蛋白编码羽扇豆醇合酶，该酶将氧化角鲨烯转化为三萜类化合物羽扇豆醇作为主要产物。羽扇豆醇合酶是一种多功能酶，它还能形成其他三萜醇，包括β-香树脂醇作为次要产物。序列分析表明，在植物与真菌和动物分化之后，羽扇豆醇合酶从环阿屯醇合酶中分化出来。这种进化顺序就是真菌和动物不能合成羽扇豆醇的原因。
