Hayashi H, Huang P, Kirakosyan A, Inoue K, Hiraoka N, Ikeshiro Y, Kushiro T, Shibuya M, Ebizuka Y
Gifu Pharmaceutical University, Japan.
Biol Pharm Bull. 2001 Aug;24(8):912-6. doi: 10.1248/bpb.24.912.
An oxidosqualene cyclase cDNA, termed GgbAS1, was isolated from cultured cells of licorice (Glycyrrhiza glabra) by heterologous hybridization with cDNA of Arabidopsis thaliana LUP1 lupeol synthase. The yeast transformed with an expression vector containing the open reading frame of GgbAS1 produced beta-amyrin, indicating that GgbAS1 encodes beta-amyrin synthase involved in the glycyrrhizin and soyasaponin biosyntheses in licorice. Northern blot analysis showed that the level of beta-amyrin synthase mRNA was drastically changed in the cultured licorice cells, whereas the mRNA level of cycloartenol synthase was relatively constant.
通过与拟南芥LUP1羽扇豆醇合酶的cDNA进行异源杂交，从甘草（Glycyrrhiza glabra）培养细胞中分离出一种氧化角鲨烯环化酶cDNA，命名为GgbAS1。用含有GgbAS1开放阅读框的表达载体转化的酵母产生了β-香树脂醇，这表明GgbAS1编码参与甘草中甘草酸和大豆皂苷生物合成的β-香树脂醇合酶。Northern印迹分析表明，培养的甘草细胞中β-香树脂醇合酶mRNA的水平发生了显著变化，而环阿屯醇合酶的mRNA水平相对恒定。
