Kałuzny J J, Szatkowski J
Kliniki Okulistycznej AM w Bydgoszczy.
Klin Oczna. 1998;100(5):315-8.
The aim of our study was to find the method of preparation of soft contact lenses for examination by SEM and next evaluation of deposits on the surfaces of frequent replacement lenses.
Frequent replacement contact lenses (Acuvue) stored in physiological saline, distilled water and sterile, dry container were prepared for examination by SEM (Novoscan 30). The best quality preparations were made from contact lenses stored in sterile, dry container and dried at room temperature. Acuvue contact lenses were taken from five young, healthy persons after one, two, three and four weeks of wearing. All the lenses were examined by SEM.
We observed nearly all the components of tear film on the surfaces of the lenses. Flat, amorphic protein, crystalline deposits, fibrinous structures were usually observed. Sometimes the nectrotic cell were seen. In one preparation we found bacteria. The amount of deposits was not high and did not increase with four weeks time of lens usage. The quality and quantity of deposits were individually variable.
The methods we choose let us to prepare good quality preparations of soft contact lenses for examination by SEM. On the surfaces of the frequent replacement contact lenses we found only a small quantity of deposits.
我们研究的目的是找到制备用于扫描电子显微镜（SEM）检查的软性隐形眼镜的方法，并对频繁更换型镜片表面的沉积物进行评估。
将频繁更换型隐形眼镜（安视优）分别储存在生理盐水、蒸馏水和无菌干燥容器中，制备用于SEM（Novoscan 30）检查的样本。质量最佳的样本是由储存在无菌干燥容器中并在室温下干燥的隐形眼镜制成的。从五名年轻健康受试者佩戴一、二、三、四周后的安视优隐形眼镜中取样。所有镜片均通过SEM检查。
我们在镜片表面观察到了几乎泪膜的所有成分。通常观察到扁平、无定形蛋白质、结晶沉积物、纤维状结构。有时可见坏死细胞。在一份样本中我们发现了细菌。沉积物的量不高，并且在镜片使用四周的时间内没有增加。沉积物的质量和数量因人而异。
我们选择的方法使我们能够制备出质量良好的软性隐形眼镜样本用于SEM检查。在频繁更换型隐形眼镜表面，我们仅发现了少量沉积物。
