Versura P, Maltarello M C, Roomans G M, Caramazza R, Laschi R
Department of Ophthalmology, University of Bologna, Italy.
Scanning Microsc. 1988 Mar;2(1):397-410.
The deposits accumulated on the surfaces of soft contact lenses are a cause of problems for the wearer of these lenses, as the deposits are never completely removed by the available washing solutions. Therefore it appears of interest to investigate the composition of these deposits. In this paper we review the major findings in the literature and, in addition, present our personal experience. We have studied new, continuously and daily worn soft contact lenses by scanning electron microscopy (SEM), X-ray microanalysis and immunohistochemistry. We have carefully evaluated preparative methods, and we can conclude that SEM and X-ray microanalysis are best carried out on unfixed, air-dried lenses. The deposits present consist mainly of mucus, especially on the tarsal side of the lenses. Chloride and potassium, coming from the tear fluid, as well as sulfur, derived from proteins, were found. Calcium was very rarely detected. IgG, IgA, IgE and C3c complement fractions were found only on the outer surfaces and not within the lens. We believe that the best characterization of the deposits is achieved by means of correlative techniques on the same lens. In fact, this approach integrates morphology and composition.
软性隐形眼镜表面积累的沉积物给佩戴者带来了问题，因为现有的清洗液无法完全清除这些沉积物。因此，研究这些沉积物的成分显得很有意义。在本文中，我们回顾了文献中的主要发现，并介绍了我们个人的经验。我们通过扫描电子显微镜（SEM）、X射线微分析和免疫组织化学研究了新的、连续每日佩戴的软性隐形眼镜。我们仔细评估了制备方法，可以得出结论，SEM和X射线微分析最好在未固定、风干的镜片上进行。存在的沉积物主要由黏液组成，尤其是在镜片的睑板一侧。发现了来自泪液的氯离子和钾离子，以及来自蛋白质的硫。很少检测到钙。IgG、IgA、IgE和C3c补体成分仅在镜片外表面发现，而不在镜片内部。我们认为，通过对同一镜片采用相关技术可以最好地表征沉积物。事实上，这种方法整合了形态学和成分分析。
