Komura S, Ueda M, Kobayashi T
Tohoku J Exp Med. 1976 Feb;118(2):173-9. doi: 10.1620/tjem.118.173.
Eight strains of male mice, C57BL, C3H SWM, SW, KK, KSB, KR and DBA, were fed on a standard pelletized diet and offered a choice of water or 10% sake solution (sake containing 10% alcohol). Both young (3 months of age) and old (8 months of age) groups were studied simultaneously. The degree of intoxication was measured by recording the drinking behavior on a pulse recorder, by calculating gaschromatographically the blood alcohol concentration, by taking depth electroencephalogram readings and so on. Intoxication, shown by lack of coordination such as grossly impaired gait, was observed only in the older mice of strain with a moderate natural alcohol preference such as C3H, SWM, SW, KK and KSB. In general, the intoxicated mice were over 9 months of age, tended to consume fluids regardless of the time of day or night and suffered a loss of body weight. The blood alcohol levels of them were over 4.6%. The threshold elevation of the ascending reticular activating system on electroencephalogram in a intoxicated mouse reached about 167%. The present study provides a successful method for the development of voluntary alcohol intoxication in mice.
选用8种品系的雄性小鼠，即C57BL、C3H、SWM、SW、KK、KSB、KR和DBA，给它们喂食标准颗粒饲料，并让它们选择饮用清水或10%的清酒溶液（含10%酒精的清酒）。同时对年轻组（3月龄）和老年组（8月龄）进行研究。通过脉搏记录仪记录饮水行为、用气相色谱法计算血液酒精浓度、读取深度脑电图等方法来测量中毒程度。只有在具有适度天然酒精偏好的品系的老年小鼠中，如C3H、SWM、SW、KK和KSB，才观察到因缺乏协调性（如步态严重受损）而表现出的中毒现象。一般来说，中毒小鼠年龄超过9个月，无论白天还是晚上都倾向于饮水，且体重减轻。它们的血液酒精水平超过4.6%。中毒小鼠脑电图中上升网状激活系统的阈值升高达到约167%。本研究为在小鼠中诱导自愿性酒精中毒提供了一种成功的方法。
