Leung P H, Salamonsen L A, Findlay J K
Prince Henry's Institute of Medical Research, Clayton, Victoria, Australia.
Hum Reprod. 1998 Dec;13(12):3469-77. doi: 10.1093/humrep/13.12.3469.
Inhibin/activin alphaC/alphaN and betaA subunits were localized immunohistochemically in the human endometrium throughout the menstrual cycle using an affinity-purified sheep polyclonal antibody raised against the alphaC/alphaN subunit and an affinity-purified rabbit polyclonal antibody raised against the betaA subunit. The betaB subunit was below the level of detection in all human endometrial samples tested. Immunoreactive inhibin alphaC/alphaN subunit was localized in the luminal epithelium, glandular epithelium, stromal tissues and vascular endothelium with no significant variation across the normal menstrual cycle. Immunoreactive betaA subunit, common to inhibin A and activins AA and AB was localized in the luminal and glandular epithelium and in migratory cells while the endometrial stromal cells, decidua, vascular smooth muscle and endothelium were devoid of immunoreactivity. A significant variation of immunoreactive betaA subunit was observed in glandular and luminal epithelium across the normal menstrual cycle. In proliferative endometrium, only a very low level of betaA immunostaining was seen in luminal and glandular epithelium, while the luminal epithelial staining increased significantly in the early secretory phase and remained relatively constant over the rest of the menstrual cycle. A progressive increase in betaA immunoreactivity was observed also in the glandular epithelium during the secretory phase reaching a maximum in the late secretory phases, and decreasing at menstruation. Co-localization studies on serial sections suggested that the migratory cells expressing strong betaA immunoreactivity were macrophages and neutrophils but not eosinophils or mast cells. Thus, cells within the human endometrium are capable of expressing inhibin/activin molecules in vivo. The variation in the pattern of secretion of the betaA subunit across the menstrual cycle suggests that activin peptides may have a physiological role in endometrial function.
使用针对αC/αN亚基的亲和纯化羊多克隆抗体和针对βA亚基的亲和纯化兔多克隆抗体，通过免疫组织化学方法在整个月经周期的人子宫内膜中定位抑制素/激活素αC/αN和βA亚基。在所有测试的人子宫内膜样本中，βB亚基低于检测水平。免疫反应性抑制素αC/αN亚基定位于腔上皮、腺上皮、基质组织和血管内皮，在正常月经周期中无显著变化。抑制素A、激活素AA和AB共有的免疫反应性βA亚基定位于腔上皮和腺上皮以及迁移细胞，而子宫内膜基质细胞、蜕膜、血管平滑肌和内皮则无免疫反应性。在正常月经周期中，腺上皮和腔上皮中观察到免疫反应性βA亚基有显著变化。在增殖期子宫内膜中，在腔上皮和腺上皮中仅可见非常低水平的βA免疫染色，而在分泌期早期腔上皮染色显著增加，并在月经周期的其余时间保持相对恒定。在分泌期，腺上皮中也观察到βA免疫反应性逐渐增加，在分泌晚期达到最大值，并在月经时下降。连续切片的共定位研究表明，表达强βA免疫反应性的迁移细胞是巨噬细胞和中性粒细胞，而不是嗜酸性粒细胞或肥大细胞。因此，人子宫内膜内的细胞能够在体内表达抑制素/激活素分子。βA亚基在月经周期中分泌模式的变化表明激活素肽可能在子宫内膜功能中具有生理作用。
