番茄叶片中苯丙氨酸解氨酶同工型的差异表达及特性
Differential expression and properties of phenylalanine ammonia-lyase isoforms in tomato leaves.
作者信息
Sarma A D, Sreelakshmi Y, Sharma R
机构信息
School of Life Sciences, University of Hyderabad, India.
出版信息
Phytochemistry. 1998 Dec;49(8):2233-43. doi: 10.1016/s0031-9422(98)00336-7.
PMID:9887524
Abstract
In tomato leaves, excision and light treatments increase phenylalanine ammonia-lyase activity, which is contributed by three PAL isoforms. These isoforms possessed similar native and subunit Mr, but were different in their pI, Km for Phe and optimal pH for activity. Also these were differentially induced and affected by metabolites belonging to particular branches of phenylpropanoid pathway.
摘要
在番茄叶片中，切除处理和光照处理会提高苯丙氨酸解氨酶的活性，该活性由三种苯丙氨酸解氨酶同工型贡献。这些同工型具有相似的天然分子量和亚基分子量，但它们的等电点、对苯丙氨酸的米氏常数以及活性的最适pH值有所不同。此外，它们受苯丙烷类途径特定分支的代谢物的诱导和影响也存在差异。
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