Chung K T, Hungate R E
Appl Environ Microbiol. 1976 Oct;32(4):649-52. doi: 10.1128/aem.32.4.649-652.1976.
A medium has been developed using alfalfa fiber as the sole substrate. It gave high culture counts (3 X 10(9) to 8 X 10(9)/ml) of rumen bacteria. When this medium was combined with the medium 98-5 of Bryant and Robinson, modified to contain 33% rumen fluid instead of 40% clarified rumen fluid, a higher count was obtained than with either medium alone.
已经开发出一种以苜蓿纤维作为唯一底物的培养基。它能使瘤胃细菌获得较高的培养数量(3×10⁹至8×10⁹/ml)。当这种培养基与经改良的布莱恩特和罗宾逊98 - 5培养基混合时,后者含有33%的瘤胃液而非40%的澄清瘤胃液,此时获得的细菌数量比单独使用任何一种培养基时都要高。