在用于计数可培养瘤胃细菌总数的培养基中添加木聚糖。
Inclusion of xylan in a medium for the enumeration of total culturable rumen bacteria.
作者信息
Henning P A, van der Walt A E
出版信息
Appl Environ Microbiol. 1978 Jun;35(6):1008-11. doi: 10.1128/aem.35.6.1008-1011.1978.
Abstract
The influence of the inclusion of xylan in a medium for enumeration of total culturable rumen bacteria was investigated. Maximum colony numbers were obtained on a medium, GCSX-2, which contained 0.033% each glucose and cellobiose and 0.067% each soluble starch and xylan. This medium gave higher colony counts than either medium 98-5 of Bryant and Robinson (J. Dairy Sci. 44:1446-1456, 1961), medium 98-5 of Chung and Hungate (Appl. Environ. Microbiol. 32:649-652, 1976), containing an added lucerne (hemicellulose + cellulose) fiber substrate, or medium GCSX-2 with the added lucerne (hemicellulose + cellulose) fraction. The time of collection of rumen fluid influenced the colony counts on the media containing the lucerne fiber substrate but was without effect on medium GCSX-2.
摘要
研究了在用于计数可培养瘤胃细菌总数的培养基中添加木聚糖的影响。在一种名为GCSX - 2的培养基上获得了最大菌落数，该培养基含有0.033%的葡萄糖和纤维二糖以及0.067%的可溶性淀粉和木聚糖。这种培养基的菌落计数高于Bryant和Robinson的98 - 5培养基（《乳品科学杂志》44:1446 - 1456, 1961）、Chung和Hungate的98 - 5培养基（《应用与环境微生物学》32:649 - 652, 1976），后者添加了苜蓿（半纤维素 + 纤维素）纤维底物，也高于添加了苜蓿（半纤维素 + 纤维素）组分的GCSX - 2培养基。采集瘤胃液的时间影响了含有苜蓿纤维底物的培养基上的菌落计数，但对GCSX - 2培养基没有影响。
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本文引用的文献
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