用于微量元素分析的细胞灰化：一种基于紫外线/臭氧的新方法。
Cell ashing for trace element analysis: A new approach based on ultraviolet/ozone.
作者信息
De Stasio G, Gilbert B, Perfetti L, Hansen R, Mercanti D, Ciotti M T, Andres R, White V E, Perfetti P, Margaritondo G
机构信息
Institut de Physique Appliquée, Ecole Polytechnique Fédérale, PH-Ecublens, Lausanne, CH-1015,
出版信息
Anal Biochem. 1999 Jan 15;266(2):174-80. doi: 10.1006/abio.1998.2901.
We studied a new approach to cell ashing based on illuminating the specimens with a low-pressure mercury discharge lamp. We analyzed with synchrotron spectromicroscopy its effects on different physiological elements in neurobiological specimens. Our results demonstrate that carbon is removed, whereas phosphorus, calcium, potassium, and sulfur are retained and their relative concentrations are enhanced. Applied to trace elements, this technique will enhance their practical detectability.
我们研究了一种基于用低压汞放电灯照射样本的细胞灰化新方法。我们用同步辐射光谱显微镜分析了其对神经生物学样本中不同生理元素的影响。我们的结果表明，碳被去除，而磷、钙、钾和硫被保留，并且它们的相对浓度增加。应用于微量元素时，该技术将提高它们的实际可检测性。
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