Seixas Sónia, Bustamante Paco, Pierce Graham J
Ciencias Exactas e Tecnologicas, Universidade Aberta, Rua Escola Politecnica, 147 1269-001 Lisbon, Portugal.
Chemosphere. 2005 May;59(8):1113-24. doi: 10.1016/j.chemosphere.2004.11.099.
Concentrations of essential (copper, iron, manganese, selenium, and zinc) and non-essential (arsenic, cadmium, mercury, and lead) trace elements were measured in arms of Octopus vulgaris. The cephalopods were sampled from commercial fishery landings at two sites on the Portuguese coast in spring 2002 and 2003. Mercury was determined using an Advanced Mercury Analyser Spectrophotometer (AMAS) and other trace elements were measured using inductively coupled plasma-atomic emission spectrometry (ICP-AES). Since high levels of arsenic were detected, identification of the forms present was carried out using high performance liquid chromatography (HPLC) followed by ICP-MS. Mean concentrations of trace elements analysed were in the following order: As > Zn > Fe > Cu >> Cd > Pb > Se > Mn >> Hg. The results of speciation of arsenic demonstrate that virtually all arsenic was in the arsenobetaine form, which is the less toxic form. The concentrations of several trace elements were generally high in samples from Viana in 2002. Cadmium concentrations were above the legal limit for human consumption in samples from Viana in 2002 and two of these animals also had lead concentrations that exceeded legal limits. Mercury appeared in all samples but levels were within legally defined safe limits. No relationship was detected between trace element concentrations and size or maturity of octopus.
对普通章鱼的腕部进行了必需微量元素（铜、铁、锰、硒和锌）和非必需微量元素（砷、镉、汞和铅）的浓度测定。这些头足类动物于2002年春季和2003年从葡萄牙海岸两个地点的商业渔业上岸处采集。汞采用高级汞分析仪分光光度计（AMAS）测定，其他微量元素采用电感耦合等离子体原子发射光谱法（ICP-AES）测定。由于检测到高含量的砷，采用高效液相色谱法（HPLC）继以电感耦合等离子体质谱法（ICP-MS）对其存在形式进行鉴定。所分析微量元素的平均浓度顺序如下：砷＞锌＞铁＞铜＞＞镉＞铅＞硒＞锰＞＞汞。砷的形态分析结果表明，几乎所有的砷都以砷甜菜碱形式存在，这是毒性较小的形式。2002年维亚纳采集的样本中几种微量元素的浓度普遍较高。2002年维亚纳采集的样本中镉浓度超过了人类食用的法定限量，其中两只动物的铅浓度也超过了法定限量。所有样本中均检测到汞，但含量在法定安全限量之内。未检测到微量元素浓度与章鱼大小或成熟度之间的关系。
