Konrad H, Gabrio T
Nahrung. 1976;20(4):395-8.
The authors developed a new enzymatic-colorimetric rapid method for testing milk for residues of organophosphorous insecticides. The test is based on the colour reaction of indoxyl acetate with the esterase of the OROSERAN (Dessau) horse serum which is inhibited by organophosphorous insecticides. Calibration curves were established for Trichlorphon and Dichlorphos (DDVP). The limit of detection lies at 0.02 mg/kg. The time actually needed per sample is about 15 min. The result is obtained within 90 min. Since this procedure requires no clean-up operations, it is labour-saving compared to other thin-layer or gas chromatographic methods. It may be recommended as a screening test for the detection of organophosphorous insecticides in milk samples.
作者开发了一种新的酶比色快速方法，用于检测牛奶中有机磷杀虫剂残留。该检测基于乙酸吲哚酚与OROSERAN（德绍）马血清酯酶的显色反应，而该酯酶会受到有机磷杀虫剂的抑制。建立了敌百虫和敌敌畏（DDVP）的校准曲线。检测限为0.02 mg/kg。每个样品实际所需时间约为15分钟。90分钟内可得到结果。由于该方法无需净化操作，与其他薄层或气相色谱法相比，节省人力。它可作为检测牛奶样品中有机磷杀虫剂的筛选试验。
