• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利:CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验

基于DNA的诊断方法的进展。

Advances in approaches to DNA-based diagnostics.

作者信息

Whitcombe D, Newton C R, Little S

机构信息

Zeneca Diagnostics Gadbrook Park Northwich Cheshire CW9 7RA UK.

出版信息

Curr Opin Biotechnol. 1998 Dec;9(6):602-8. doi: 10.1016/s0958-1669(98)80137-7.

DOI:10.1016/s0958-1669(98)80137-7
PMID:9889145
Abstract

The most tangible advances in DNA diagnostics during the past year have been in enhancing existing techniques to simplify their use and improve throughput. This has led to simplified genotyping methods using homogeneous analysis coupled with spectral data output. Miniaturisation and increased throughput have also been achieved through improvements in DNA chip technology.

摘要

过去一年里,DNA诊断领域最切实的进展在于改进现有技术,以简化其使用方法并提高通量。这催生了采用均相分析结合光谱数据输出的简化基因分型方法。通过改进DNA芯片技术,还实现了小型化和通量增加。

相似文献

1
Advances in approaches to DNA-based diagnostics.基于DNA的诊断方法的进展。
Curr Opin Biotechnol. 1998 Dec;9(6):602-8. doi: 10.1016/s0958-1669(98)80137-7.
2
Biotechnology and molecular diagnostics.生物技术与分子诊断
Forum (Genova). 1999 Jul-Dec;9(3 Suppl 3):37-46.
3
Pitfalls of PCR-based strategy for genotyping cre-loxP mice.基于PCR的cre-loxP小鼠基因分型策略的陷阱
Biotechniques. 2007 Mar;42(3):281, 283. doi: 10.2144/000112429.
4
A reliable method for the removal of co-purifying PCR inhibitors from ancient DNA.
Biotechniques. 1994 Feb;16(2):232-5.
5
Retrieval of flanking DNA using a PCR-based approach with restriction enzyme-digested genomic DNA template.
Biotechniques. 1999 Apr;26(4):630-2, 634. doi: 10.2144/99264bm08.
6
Introduction to recombinant-DNA technology.重组DNA技术简介。
Am J Clin Nutr. 1993 Aug;58(2 Suppl):249S-258S. doi: 10.1093/ajcn/58.2.249S.
7
Rapid and simple purification of PCR products by direct band elution during agarose gel electrophoresis.通过琼脂糖凝胶电泳期间的直接条带洗脱快速简便地纯化PCR产物。
Biotechniques. 1993 Jan;14(1):28-30.
8
Principles and applications of polymerase chain reaction: basic science for the practicing physician.聚合酶链反应的原理与应用:执业医师的基础科学
Ann Allergy Asthma Immunol. 2008 Oct;101(4):437-43. doi: 10.1016/S1081-1206(10)60323-7.
9
Instrumentation in molecular biomedical diagnostics: an overview.
Genet Anal. 1997 Jul;14(2):31-6. doi: 10.1016/s1050-3862(97)00006-5.
10
Extender PCR: a method for the isolation of sequences regulating gene expression from genomic DNA.延伸聚合酶链反应:一种从基因组DNA中分离调控基因表达序列的方法。
Biotechniques. 1999 May;26(5):804-6. doi: 10.2144/99265bm01.

引用本文的文献

1
Rapid and quantitative detection of Shiga toxin1 and Shiga toxin2 based on multiple targets UPT-LF assay.基于多靶点通用型光激化学发光免疫分析的志贺毒素1和志贺毒素2快速定量检测
Eng Life Sci. 2020 Jul 19;20(11):494-503. doi: 10.1002/elsc.202000031. eCollection 2020 Nov.
2
Rapid Detection and Identification of Uveitis Pathogens by Qualitative Multiplex Real-Time PCR.定性多重实时 PCR 快速检测和鉴定葡萄膜炎病原体。
Invest Ophthalmol Vis Sci. 2018 Jan 1;59(1):582-589. doi: 10.1167/iovs.17-22597.
3
Sensitive sequencing method for KRAS mutation detection by Pyrosequencing.
焦磷酸测序法检测KRAS突变的灵敏测序方法
J Mol Diagn. 2005 Aug;7(3):413-21. doi: 10.1016/S1525-1578(10)60571-5.
4
[Papillomavirus diseases].[乳头瘤病毒疾病]
Hautarzt. 2004 Sep;55(9):841-54. doi: 10.1007/s00105-004-0795-3.
5
Tripartite molecular beacons.三方分子信标
Nucleic Acids Res. 2002 Sep 15;30(18):e94. doi: 10.1093/nar/gnf093.
6
Quantitative detection of single nucleotide polymorphisms for a pooled sample by a bioluminometric assay coupled with modified primer extension reactions (BAMPER).通过结合改良引物延伸反应的生物发光测定法(BAMPER)对混合样本中的单核苷酸多态性进行定量检测。
Nucleic Acids Res. 2001 Oct 1;29(19):E93. doi: 10.1093/nar/29.19.e93.
7
DNA microarrays with stem-loop DNA probes: preparation and applications.带有茎环DNA探针的DNA微阵列:制备与应用
Nucleic Acids Res. 2001 Oct 1;29(19):E92. doi: 10.1093/nar/29.19.e92.
8
Direct detection of Legionella species from bronchoalveolar lavage and open lung biopsy specimens: comparison of LightCycler PCR, in situ hybridization, direct fluorescence antigen detection, and culture.从支气管肺泡灌洗和开胸肺活检标本中直接检测军团菌属:LightCycler聚合酶链反应、原位杂交、直接荧光抗原检测和培养的比较
J Clin Microbiol. 2001 Jul;39(7):2618-26. doi: 10.1128/JCM.39.7.2618-2626.2001.
9
K-Ras mutations and benign pancreatic disease.K-Ras基因突变与胰腺良性疾病
Int J Pancreatol. 2000 Apr;27(2):93-103. doi: 10.1385/IJGC:27:2:093.
10
Digital PCR.数字PCR
Proc Natl Acad Sci U S A. 1999 Aug 3;96(16):9236-41. doi: 10.1073/pnas.96.16.9236.