Mollenhauer J, Mok M T, King K B, Gupta M, Chubinskaya S, Koepp H, Cole A A
Department of Biochemistry, Rush Medical College at Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612, USA.
J Histochem Cytochem. 1999 Feb;47(2):209-20. doi: 10.1177/002215549904700209.
In its tissue-specific function as a collagen receptor of chondrocytes, cartilage annexin V (anchorin CII) occupies a key position in the organization of the cell-extracellular matrix (ECM) junction for the tissue. The general role of annexin V (Anx V) in other tissues suggests involvement in cellular secretory processes and in regulation of apoptosis. Immunohistochemical analysis of Anx V in growth plate cartilage, confirmed by in situ hybridization, suggests that Anx V is prominently expressed and forms a major constituent of growth plate chondrocytes. Anx V epitopes are also located in the pericellular matrix of hypertrophic cartilage. In adult articular cartilage the expression is downregulated, with the highest levels of immunostaining found in the upper third of the articular cartilage layers and almost no antigen found in the deep layers. Osteoarthritic (OA) cartilage is characterized by a significant upregulation of message and protein throughout the entire depth of the tissue, an accumulation of cytoplasmic annexin V epitopes, and a release of epitopes into the pericellular and interterritorial matrix, in part co-localized with granular structures. Therefore, Anx V expression and tissue distribution may serve as a histological marker for metabolic alterations and for changes in the cellular phenotype associated with OA.
作为软骨细胞的胶原蛋白受体，软骨膜联蛋白V（锚定蛋白CII）在该组织的细胞 - 细胞外基质（ECM）连接组织中占据关键位置。膜联蛋白V（Anx V）在其他组织中的一般作用表明其参与细胞分泌过程和细胞凋亡的调节。通过原位杂交证实，对生长板软骨中Anx V的免疫组织化学分析表明，Anx V显著表达并构成生长板软骨细胞的主要成分。Anx V表位也位于肥大软骨的细胞周围基质中。在成人关节软骨中，表达下调，在关节软骨层的上三分之一中发现最高水平的免疫染色，而在深层几乎没有发现抗原。骨关节炎（OA）软骨的特征是在组织的整个深度范围内，信息和蛋白质显著上调，细胞质膜联蛋白V表位积累，并且表位释放到细胞周围和区域间基质中，部分与颗粒结构共定位。因此，Anx V的表达和组织分布可作为代谢改变以及与OA相关的细胞表型变化的组织学标志物。
