Koonjul P K, Brandt W F, Farrant J M, Lindsey G G
Department of Biochemistry and Department of Botany, University of Cape Town, Private Bag, Rondebosch 7701, South Africa.
Nucleic Acids Res. 1999 Feb 1;27(3):915-6. doi: 10.1093/nar/27.3.915.
Polysaccharides, secondary metabolites and poly-phenolics are known to co-isolate with nucleic acids from plant tissues resulting in inhibition of molecular manipulations. RNA isolated from the polyphenolic-rich resurrection plant, Myrothamnus flabellifolius, was demonstrated to inhibit a standard polymerase chain reaction used as an assay despite the inclusion of the polyphenolic-binding compound poly(1-vinylpyrrolidone-2) (PVP) into the RNA isolation medium. This inhibition was, however, reversed by the addition of PVP into the PCR mixture itself. Confirmation of the inhibitory effect of polyphenolics on PCR was obtained by addition of green tea polyphenolics to the standard PCR assay. This inhibition was also reversed by the simultaneous inclusion of PVP.
已知多糖、次生代谢产物和多酚会与植物组织中的核酸共分离，从而抑制分子操作。从富含多酚的复苏植物扇叶鼠李中分离出的RNA，尽管在RNA分离介质中加入了多酚结合化合物聚（1-乙烯基吡咯烷酮-2）（PVP），但仍被证明会抑制用作检测的标准聚合酶链反应。然而，通过在PCR混合物本身中加入PVP，这种抑制作用得以逆转。通过向标准PCR检测中添加绿茶多酚，证实了多酚对PCR的抑制作用。同时加入PVP也可逆转这种抑制作用。
