AP-1和T3RE顺式元件在人类苹果酸酶基因的转录调控中作为一个功能单元发挥作用。

AP-1 and T3RE cis elements operate as a functional unit in the transcriptional control of the human malic enzyme gene.

作者信息

González-Manchón C, Ayuso M S, Parrilla R

机构信息

Department of Pathophysiology and Human Molecular Genetics, Centro de Investigaciones Biológicas (CSIC), Velázquez 144, 28006, Madrid, Spain.

出版信息

Gene. 1999 Jan 8;226(1):111-9. doi: 10.1016/s0378-1119(98)00543-5.

DOI:10.1016/s0378-1119(98)00543-5

PMID:9889342

Abstract

The human malic enzyme (hME) promoter contains an inverted palindromic (IP4) 3,5,3'-triiodo-thyronine (T3) response element (T3RE) 15bp downstream from an activating protein-1 (AP-1) site. The purpose of this study was to analyze the functional relationship between both cis-acting elements. The following observations indicate that these two elements operate as a functional unit in controlling the human ME gene:T3 failed to stimulate transcription above the basal levels in cells overexpressing either TRb or TRb/retinoid acid receptor (RXR), indicating that TRbeta acts primarily as a transcriptional repressor in the context of the hME. Moreover, the finding of a repressive effect of TRbeta without DNA binding suggests the existence of both DNA-dependent and independent mechanisms of TRbeta-induced repression of transcription.

摘要

人苹果酸酶（hME）启动子在激活蛋白-1（AP-1）位点下游15bp处含有一个反向回文（IP4）3,5,3'-三碘甲状腺原氨酸（T3）反应元件（T3RE）。本研究的目的是分析这两个顺式作用元件之间的功能关系。以下观察结果表明，这两个元件在控制人ME基因时作为一个功能单元发挥作用：在过表达TRβ或TRβ/视黄酸受体（RXR）的细胞中，T3未能刺激转录水平超过基础水平，这表明在hME的背景下，TRβ主要作为转录抑制因子发挥作用。此外，TRβ无DNA结合时具有抑制作用这一发现表明，存在TRβ诱导转录抑制的DNA依赖性和非依赖性机制。