Forman S, Kás J, Fini F, Steinberg M, Ruml T
Department of Biochemistry and Microbiology, Institute of Chemical Technology, Prague, Czech Republic.
J Biochem Mol Toxicol. 1999;13(1):11-5. doi: 10.1002/(sici)1099-0461(1999)13:1<11::aid-jbt2>3.0.co;2-r.
Testing of the effects of xenobiotics in cultured cells often requires the use of organic solvents to effect suspension of the test agents in cell culture media. However, the toxic effects of the solvents themselves may introduce artifacts, which obscure interpretation of the experimental results. In this article, the toxicity of different solvents commonly used for solvation of a variety of xenobiotic agents was studied. We show that ethanol, acetone, isooctane, methanol, and hexane were considerably less toxic than the more commonly used solvent, DMSO, when ATP content and growth rates of HeLa cells exposed to these solvents was measured.
在培养细胞中测试外源化合物的效应通常需要使用有机溶剂，以使测试剂在细胞培养基中悬浮。然而，溶剂本身的毒性作用可能会引入假象，从而模糊实验结果的解读。在本文中，我们研究了常用于溶解多种外源化合物的不同溶剂的毒性。当测量暴露于这些溶剂的HeLa细胞的ATP含量和生长速率时，我们发现乙醇、丙酮、异辛烷、甲醇和己烷的毒性明显低于更常用的溶剂二甲基亚砜（DMSO）。
