儿童中抗细胞毒素相关基因A（CagA）抗体的流行率并非特定疾病的标志物。

The prevalence of antibody to CagA in children is not a marker for specific disease.

作者信息

Mitchell H M, Hazell S L, Bohane T D, Hu P, Chen M, Li Y Y

机构信息

The School of Microbiology and Immunology, University of New South Wales, Sydney, Australia.

出版信息

J Pediatr Gastroenterol Nutr. 1999 Jan;28(1):71-5. doi: 10.1097/00005176-199901000-00016.

DOI:10.1097/00005176-199901000-00016

PMID:9890472

Abstract

BACKGROUND

In adults, a high prevalence of antibody to the cytotoxin-associated antigen (CagA) of Helicobacter pylori has been linked to the development of more serious gastroduodenal disease. Few investigators have examined this association in children. The purpose of this study was to investigate the seroprevalence of antibody to the CagA antigen as well as other specific H. pylori antigens in children.

METHODS

By use of an immunoblot analysis kit, the immune response to specific H. pylori antigens in serum collected from 21 H. pylori-positive symptomatic Australian children, 5 with peptic ulcer disease and 16 with nonulcer dyspepsia, and 33 H. pylori-positive asymptomatic Chinese children. Sera from 20 H. pylori-negative symptomatic Australian children were used as control subjects.

RESULTS

Antibody responses to the 26.5-kDa, 30-kDa, and 116-kDa (CagA) antigens were found to be the most prevalent, with 81.5%, 79.6%, and 76% of children, respectively, mounting a response. In contrast, antibody responses to the 19.5-kDa, 35-kDa, 45-kDa, 60-kDa, 89 kDa (VacA), and 180-kDa antigens occurred in 55.5%, 24%, 16.7%, 63%, 37%, and 7.4% of children, respectively. A higher prevalence of antibody response to CagA was found in the symptomatic Australian children with peptic ulcer disease (100%) compared with prevalence in those with nonulcer dyspepsia (56.3%), but the difference did not reach statistical significance. No significant difference was found between the prevalence of antibody to CagA in the Australian peptic ulcer disease group (100%) and that in the asymptomatic Chinese children (81.8%).

CONCLUSION

These results suggest that in children CagA is not a marker of specific disease development.

摘要

背景

在成人中，幽门螺杆菌细胞毒素相关抗原（CagA）抗体的高流行率与更严重的胃十二指肠疾病的发生有关。很少有研究者在儿童中研究这种关联。本研究的目的是调查儿童中CagA抗原以及其他幽门螺杆菌特异性抗原的血清流行率。

方法

使用免疫印迹分析试剂盒，检测从21名幽门螺杆菌阳性的有症状澳大利亚儿童（5名患有消化性溃疡疾病，16名患有非溃疡性消化不良）和33名幽门螺杆菌阳性的无症状中国儿童采集的血清中对幽门螺杆菌特异性抗原的免疫反应。将20名幽门螺杆菌阴性的有症状澳大利亚儿童的血清用作对照。

结果

发现对26.5 kDa、30 kDa和116 kDa（CagA）抗原的抗体反应最为普遍，分别有81.5%、79.6%和76%的儿童产生了反应。相比之下，对19.5 kDa、35 kDa、45 kDa、60 kDa、89 kDa（VacA）和180 kDa抗原的抗体反应分别发生在55.5%、24%、16.7%、63%、37%和7.4%的儿童中。与患有非溃疡性消化不良的儿童（56.3%）相比，患有消化性溃疡疾病的有症状澳大利亚儿童中对CagA的抗体反应患病率更高（100%），但差异未达到统计学意义。澳大利亚消化性溃疡疾病组（100%）和无症状中国儿童组（81.8%）中CagA抗体的患病率之间没有显著差异。