Ayres H M, Furr J R, Russell A D
Welsh School of Pharmacy, Cardiff University, UK.
Lett Appl Microbiol. 1998 Dec;27(6):372-4. doi: 10.1046/j.1472-765x.1998.00455.x.
The presence of divalent (Mg2+) ions greatly reduced the lysis of Pseudomonas aeruginosa strain G48 in a system at pH 7.8 or 9.0 consisting of ethylenediamine tetraacetic acid (EDTA), lysozyme and tris. Similar reductions in lysis occurred when EDTA was replaced by nitrilotriacetic acid, sodium citrate or sodium polyphosphate. The effect depended on the cation concentration. Mg2+ may replace cations removed from the outer membrane, or may effectively remove the permeabilizer from the system. The results suggest that the permeabilizing activity associated with these agents against this organism has a common basis in affecting the outer membrane.
在pH 7.8或9.0、由乙二胺四乙酸（EDTA）、溶菌酶和三羟甲基氨基甲烷组成的体系中，二价（Mg2+）离子的存在极大地降低了铜绿假单胞菌G48菌株的裂解。当EDTA被次氮基三乙酸、柠檬酸钠或多磷酸钠取代时，也会出现类似的裂解减少情况。这种效应取决于阳离子浓度。Mg2+可能替代从外膜去除的阳离子，或者可能有效地从体系中去除通透剂。结果表明，这些试剂对该生物体的通透活性在影响外膜方面有共同的基础。
