Mikami Y, Hata S, Melamed J, Moriya T, Manabe T
Department of Pathology, Kawasaki Medical School, Okayama, Japan.
Int J Gynecol Pathol. 1999 Jan;18(1):52-7. doi: 10.1097/00004347-199901000-00007.
Stromal hyalinization in ovarian clear cell carcinomas has been suggested to be caused by deposition of basement membrane (BM) material, but the biological and diagnostic significance of this finding remains unknown. The distribution of BM material in 17 primary ovarian clear cell carcinomas was examined semiquantitatively using hematoxylin and eosin-stained sections and immunohistochemistry with antibodies to laminin and type IV collagen. For comparison, other surface epithelial tumors, including 8 serous tumors of low malignant potential, 10 serous adenocarcinomas, 6 mucinous tumors of low malignant potential, 5 mucinous adenocarcinomas, 6 endometrioid carcinomas, 4 Brenner tumors, 1 transitional cell carcinoma, and 3 undifferentiated carcinomas, were examined. Stromal hyalinization was found in all 17 clear cell carcinomas and was immunoreactive for type IV collagen and laminin. Other types of surface epithelial tumor lacked these findings. In clear cell carcinoma, areas showing a papillary pattern tended to show abundant deposition regardless of nuclear grade, whereas in solid, tubular, or cystic areas, the deposition was more prominent in areas showing high-nuclear-grade features (grade 2 and 3) than in areas with low-nuclear-grade features (grade 1). Dense deposition of BM material recognized as stromal hyalinization on hematoxylin and eosin-stained sections in primary ovarian clear cell carcinoma is a characteristic feature that is not seen in other ovarian surface epithelial tumors. This matrix production correlates with high-nuclear-grade features and papillary growth pattern.
卵巢透明细胞癌中的间质玻璃样变被认为是由基底膜（BM）物质沉积所致，但这一发现的生物学及诊断意义仍不明确。使用苏木精-伊红染色切片及针对层粘连蛋白和IV型胶原的抗体进行免疫组织化学，对17例原发性卵巢透明细胞癌中BM物质的分布进行了半定量检测。作为对照，还检测了其他表面上皮性肿瘤，包括8例低恶性潜能浆液性肿瘤、10例浆液性腺癌、6例低恶性潜能黏液性肿瘤、5例黏液性腺癌、6例子宫内膜样癌、4例勃勒纳瘤、1例移行细胞癌及3例未分化癌。在所有17例透明细胞癌中均发现了间质玻璃样变，且对IV型胶原和层粘连蛋白呈免疫反应性。其他类型的表面上皮性肿瘤未出现这些表现。在透明细胞癌中，呈乳头状模式的区域无论核分级如何往往都有大量沉积，而在实性、管状或囊性区域，高核分级特征（2级和3级）区域的沉积比低核分级特征（1级）区域更为显著。原发性卵巢透明细胞癌中在苏木精-伊红染色切片上被识别为间质玻璃样变的BM物质致密沉积是一种特征性表现，在其他卵巢表面上皮性肿瘤中未见。这种基质产生与高核分级特征及乳头状生长模式相关。
