Koomägi R, Sauerbrey A, Zintl F, Volm M
German Cancer Research Center, Department of Oncological Diagnostics and Therapy, Heidelberg, Germany.
Anticancer Res. 1998 Nov-Dec;18(6A):4307-9.
In a retrospective study, immunocytochemistry was used to analyze the cells of 62 newly diagnosed and 28 relapsed childhood acute lymphoblastic leukemias (ALL) for the expression of nm23-H1 protein. Of the 62 newly diagnosed ALL, only 9 cases exhibited positive staining (15%) while 10 of the 28 relapsed ALL did (36%). This difference is statistically significant (P = 0.03, Fisher's exact test). Furthermore, the presence of mutations in the exons 1-5 was investigated by RT-PCR and single-strand conformation polymorphism (SSCP) analysis. No mutations could be detected in either the newly diagnosed or relapsed ALL. This finding suggests that nm23-H1 mutations may be a rare event in ALL.
在一项回顾性研究中，采用免疫细胞化学方法分析了62例新诊断的和28例复发的儿童急性淋巴细胞白血病（ALL）细胞中nm23-H1蛋白的表达情况。在62例新诊断的ALL中，只有9例呈阳性染色（15%），而28例复发ALL中有10例呈阳性染色（36%）。这种差异具有统计学意义（P = 0.03，Fisher精确检验）。此外，通过逆转录聚合酶链反应（RT-PCR）和单链构象多态性（SSCP）分析研究了外显子1-5中突变的存在情况。在新诊断的或复发的ALL中均未检测到突变。这一发现表明，nm23-H1突变在ALL中可能是罕见事件。
