Lehrer M
Department of Pathology, Long Island Jewish Medical Center, New Hyde Park, New York, USA.
Clin Lab Med. 1998 Dec;18(4):631-49.
Appropriate GC/MS analytic techniques can provide conclusive confirmatory evidence for the presence of drugs and metabolites in forensic urine drug testing. Although forensic laboratories are required to utilize GC/MS, they have flexibility and freedom in selecting instrumentation, modes of operation, and actual analytic methods. It is incumbent on the laboratory to optimize analytic procedures so that reliability and accuracy are maximized. The laboratory needs to be aware of potential pitfalls in both the handling and the analyses of specimens. Specificity can be enhanced by utilizing mass spectrometric operating modes such as CI and full scan, which serve to complement SIM analyses. They are useful in solving difficult identification problems and in enhancing sensitivity as well as specificity. Newer types of GC/MS systems utilizing ion trapping or tandem mass spectrometry offer the promise of enhanced analytic performance and productivity gains. Knowledge of the advantages and potential pitfalls of different GC/MS systems as well as ionization and detection modes is helpful in optimizing the accuracy of compound identification.
合适的气相色谱/质谱（GC/MS）分析技术可为法医尿液药物检测中药物和代谢物的存在提供确凿的确认证据。尽管法医实验室被要求使用GC/MS，但它们在选择仪器、操作模式和实际分析方法方面具有灵活性和自主性。实验室有责任优化分析程序，以使可靠性和准确性最大化。实验室需要意识到样本处理和分析过程中的潜在陷阱。通过使用诸如化学电离（CI）和全扫描等质谱操作模式可以提高特异性，这些模式有助于补充选择离子监测（SIM）分析。它们有助于解决困难的鉴定问题，并提高灵敏度和特异性。采用离子阱或串联质谱的新型GC/MS系统有望提高分析性能并提高工作效率。了解不同GC/MS系统以及电离和检测模式的优点和潜在陷阱有助于优化化合物鉴定的准确性。
