通过精氨酸tRNA（CCG）的过表达对大肠杆菌rnpA49突变体生长缺陷的互补作用
Complementation of the growth defect of an rnpA49 mutant of Escherichia coli by overexpression of arginine tRNA(CCG).
作者信息
Kim M S, Park B H, Kim S, Lee Y J, Chung J H, Lee Y
机构信息
Department of Chemistry, Korea Advanced Institute of Science and Technology, Taejon, Korea.
出版信息
Biochem Mol Biol Int. 1998 Dec;46(6):1153-60. doi: 10.1080/15216549800204712.
We previously found that overexpression of arginine tRNA(CCG) from Brevibacterium albidum complements the rnpA49 mutation, which is responsible for the thermosensitivity of Escherichia coli RNase P function. In this present work, we show that the E. coli homologue tRNA also complements the same mutation, but other tRNAs do not. These results suggest that the rnpA49 mutation causes a major cellular defect in an RNase P reaction to generate the mature arginine tRNA(CCG).
我们之前发现，来自白色短杆菌的精氨酸tRNA（CCG）的过表达可弥补rnpA49突变，该突变导致大肠杆菌核糖核酸酶P功能的温度敏感性。在本研究中，我们表明大肠杆菌同源tRNA也能弥补相同的突变，但其他tRNA则不能。这些结果表明，rnpA49突变在核糖核酸酶P反应中产生成熟精氨酸tRNA（CCG）时导致了主要的细胞缺陷。
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